An expanded set of fluorogenic sulfatase activity probes

Elizabeth L. Smith, Carolyn R. Bertozzi, Kimberly E. Beatty

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases. Revealing sulfatases: Two new fluorogenic enzyme probes, 3-O-methylfluorescein sulfate and resorufin-sulfate, display a large increase in quantum yield upon hydrolysis by sulfatases. Both probes enable rapid, sensitive detection of sulfatase activity in a protein gel assay format, which allows the examination of sulfatases from a variety of mycobacterial pathogens.

Original languageEnglish (US)
Pages (from-to)1101-1105
Number of pages5
Issue number8
StatePublished - May 26 2014


  • Mycobacterium tuberculosis
  • fluorescent probes
  • hydrolases
  • protein imaging
  • sulfatases

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry


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