An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase

Richard Longnecker, Brian Druker, Thomas M. Roberts, Elliott Kieff

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) encodes two integral membrane proteins in latently infected growth-transformed cells. One of these, LMP1, can transform rodent fibroblasts and induce markers of B-lymphocyte activation. The second, LMP2, colocalizes with LMP1 in a constitutive patch in the EBV-transformed B-lymphocyte plasma membrane. The experiments reported here demonstrate that LMP2 may biochemically interact with LMP1 and that LMP2 closely associates with and is an important substrate for a B-lymphocyte tyrosine kinase in EBV-transformed B lymphocytes or in B-lymphoma cells in which LMP2 is expressed by gene transfer. LMP2 is also serine and threonine phosphorylated. LMP2 localizes to a peripheral membrane (presumably plasma membrane) patch in transfected B-lymphoma cells and colocalizes with much of the cellular tyrosine-phosphorylated proteins. LMP2 undergoes tyrosine phosphorylation in anti-LMP2 or antiphosphotyrosine immunoprecipitates from transfected B-lymphoma cells or EBV-transformed B lymphocytes. The first 167 of the 497 amino acids of LMP2 retain full ability to associate with and act as a substrate for a tyrosine kinase. A 70-kDa phosphotyrosine cell protein associates with LMP2 in transfected cells or in EBV-transformed B lymphocytes and could be a mediator of the effects of LMP2.

Original languageEnglish (US)
Pages (from-to)3681-3692
Number of pages12
JournalJournal of Virology
Volume65
Issue number7
StatePublished - Jul 1991
Externally publishedYes

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Human herpesvirus 4
Human Herpesvirus 4
Protein-Tyrosine Kinases
B-lymphocytes
tyrosine
cell growth
phosphotransferases (kinases)
B-Lymphocytes
B-Cell Lymphoma
Growth
lymphoma
Proteins
proteins
cells
Tyrosine
plasma membrane
Cell Membrane
Phosphotyrosine
Threonine
lymphocyte proliferation

ASJC Scopus subject areas

  • Immunology

Cite this

An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase. / Longnecker, Richard; Druker, Brian; Roberts, Thomas M.; Kieff, Elliott.

In: Journal of Virology, Vol. 65, No. 7, 07.1991, p. 3681-3692.

Research output: Contribution to journalArticle

Longnecker, Richard ; Druker, Brian ; Roberts, Thomas M. ; Kieff, Elliott. / An Epstein-Barr virus protein associated with cell growth transformation interacts with a tyrosine kinase. In: Journal of Virology. 1991 ; Vol. 65, No. 7. pp. 3681-3692.
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