An elution procedure for visualization of adult hemoglobins in human blood smears

David Kabat

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A procedure is described for visualization of normal and mutant adult hemoglobins in human blood smears. After extraction of blood smears with a concentrated potassium phosphate buffer (2.76 M, pH 7.2), erythrocytes that had adult hemoglobins stained bright red with erythrosin, whereas cells that had only fetal hemoglobin appeared as clear ghosts. Analyses of cord blood from newborn infants indicate that, although most erythrocytes contain obly Hb F and a few contain only Hb A, many contain both hemoglobins A and F.

Original languageEnglish (US)
Pages (from-to)239-242
Number of pages4
JournalBlood
Volume43
Issue number2
StatePublished - 1974

Fingerprint

Fetal Hemoglobin
Hemoglobins
Blood
Visualization
Erythrosine
Erythrocytes
Hemoglobin A
Fetal Blood
Buffers
Newborn Infant
potassium phosphate

ASJC Scopus subject areas

  • Hematology

Cite this

An elution procedure for visualization of adult hemoglobins in human blood smears. / Kabat, David.

In: Blood, Vol. 43, No. 2, 1974, p. 239-242.

Research output: Contribution to journalArticle

Kabat, David. / An elution procedure for visualization of adult hemoglobins in human blood smears. In: Blood. 1974 ; Vol. 43, No. 2. pp. 239-242.
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