TY - JOUR
T1 - An automated system for the mapping and quantitative analysis of immunocytochemistry of an inducible nuclear protein
AU - Cecchi, Guillermo A.
AU - Ribeiro, Sidarta
AU - Mello, Claudio V.
AU - Magnasco, Marcelo O.
N1 - Funding Information:
We thank A. Alvarez-Buylla for lending his microscope in the initial phases of the project, and Bozenka Glatt and Kostadinka Arissian for helping in the aquisition of our optical table. Work supported by the Mathers Foundation (MM), Kluge and CNPq Fellowships (SR) and NIDCD Grant #02853 (CVM).
PY - 1999/3/1
Y1 - 1999/3/1
N2 - We describe here an automated system that accurately maps tissue sections stained by immunocytochemistry for an inducible nuclear protein. The sections are scanned with a computer-controlled microscope setup hooked to a CCD camera. Raw images captured at high resolution are filtered using highly selective criteria for the recognition of labeled cell nuclei. The total population of recognized labeled nuclei is then divided into separate bins, according to their labeling intensities. Finally, information about both the position and labeling intensity of labeled nuclei is represented in average density maps. The system was optimized for the quantitative mapping of neuronal cells expressing the inducible gene ZENK in the brain of songbirds, in response to stimulation with song, but should be of general applicability for the mapping of inducible nuclear proteins.
AB - We describe here an automated system that accurately maps tissue sections stained by immunocytochemistry for an inducible nuclear protein. The sections are scanned with a computer-controlled microscope setup hooked to a CCD camera. Raw images captured at high resolution are filtered using highly selective criteria for the recognition of labeled cell nuclei. The total population of recognized labeled nuclei is then divided into separate bins, according to their labeling intensities. Finally, information about both the position and labeling intensity of labeled nuclei is represented in average density maps. The system was optimized for the quantitative mapping of neuronal cells expressing the inducible gene ZENK in the brain of songbirds, in response to stimulation with song, but should be of general applicability for the mapping of inducible nuclear proteins.
KW - Immunocytochemistry
KW - Inducible nuclear protein
KW - Mapping
KW - Quantitative analysis
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U2 - 10.1016/S0165-0270(98)00179-4
DO - 10.1016/S0165-0270(98)00179-4
M3 - Article
C2 - 11230811
AN - SCOPUS:0033106190
SN - 0165-0270
VL - 87
SP - 147
EP - 158
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 2
ER -