An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species

A. C. Switchenko, C. G. Miyada, T. C. Goodman, T. J. Walsh, Brian Wong, M. J. Becker, E. F. Ullman

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

An automated enzymatic method was developed for the measurement of D- arabinitol in human serum. The assay is based on a novel, highly specific D- arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH produced is used in a second reaction to reduce p- iodonitrotetrazolium violet (INT) to INT-formazan, which is measured spectrophotometrically. The entire reaction sequence can be performed automatically on a COBAS MIRA-S clinical chemistry analyzer (Roche Diagnostic Systems, Inc., Montclair, N.J.). Replicate analyses of human sera supplemented with D-arabinitol over a concentration range of 0 to 40 μM demonstrated that the pentitol could be measured with an accuracy of ±7% and a precision (standard deviation) of ±0.4 μM. Serum D-arabinitol measurements correlated with those determined by gas chromatography (r = 0.94). The enzymatic method is unaffected by L-arabinitol, D-mannitol, or other polyols commonly found in human serum. Any of 17 therapeutic drugs potentially present in serum did not significantly influence assay performance. Data illustrating the application of the assay in patients for possible diagnosis of invasive candidiasis and the monitoring of therapeutic intervention are presented. The automated assay described here was developed to facilitate the investigation of D-arabinitol as a serum marker for invasive Candida infections.

Original languageEnglish (US)
Pages (from-to)92-97
Number of pages6
JournalJournal of Clinical Microbiology
Volume32
Issue number1
StatePublished - 1994
Externally publishedYes

Fingerprint

Candida
NAD
Serum
D-arabinitol dehydrogenase
Invasive Candidiasis
Formazans
Candida tropicalis
Clinical Chemistry
Mannitol
Gas Chromatography
arabitol
Biomarkers
Enzymes
Therapeutics
Infection
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Switchenko, A. C., Miyada, C. G., Goodman, T. C., Walsh, T. J., Wong, B., Becker, M. J., & Ullman, E. F. (1994). An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species. Journal of Clinical Microbiology, 32(1), 92-97.

An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species. / Switchenko, A. C.; Miyada, C. G.; Goodman, T. C.; Walsh, T. J.; Wong, Brian; Becker, M. J.; Ullman, E. F.

In: Journal of Clinical Microbiology, Vol. 32, No. 1, 1994, p. 92-97.

Research output: Contribution to journalArticle

Switchenko, AC, Miyada, CG, Goodman, TC, Walsh, TJ, Wong, B, Becker, MJ & Ullman, EF 1994, 'An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species', Journal of Clinical Microbiology, vol. 32, no. 1, pp. 92-97.
Switchenko AC, Miyada CG, Goodman TC, Walsh TJ, Wong B, Becker MJ et al. An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species. Journal of Clinical Microbiology. 1994;32(1):92-97.
Switchenko, A. C. ; Miyada, C. G. ; Goodman, T. C. ; Walsh, T. J. ; Wong, Brian ; Becker, M. J. ; Ullman, E. F. / An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species. In: Journal of Clinical Microbiology. 1994 ; Vol. 32, No. 1. pp. 92-97.
@article{2e187a0d29ee4cb6adc14ca4f248d9b6,
title = "An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species",
abstract = "An automated enzymatic method was developed for the measurement of D- arabinitol in human serum. The assay is based on a novel, highly specific D- arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH produced is used in a second reaction to reduce p- iodonitrotetrazolium violet (INT) to INT-formazan, which is measured spectrophotometrically. The entire reaction sequence can be performed automatically on a COBAS MIRA-S clinical chemistry analyzer (Roche Diagnostic Systems, Inc., Montclair, N.J.). Replicate analyses of human sera supplemented with D-arabinitol over a concentration range of 0 to 40 μM demonstrated that the pentitol could be measured with an accuracy of ±7{\%} and a precision (standard deviation) of ±0.4 μM. Serum D-arabinitol measurements correlated with those determined by gas chromatography (r = 0.94). The enzymatic method is unaffected by L-arabinitol, D-mannitol, or other polyols commonly found in human serum. Any of 17 therapeutic drugs potentially present in serum did not significantly influence assay performance. Data illustrating the application of the assay in patients for possible diagnosis of invasive candidiasis and the monitoring of therapeutic intervention are presented. The automated assay described here was developed to facilitate the investigation of D-arabinitol as a serum marker for invasive Candida infections.",
author = "Switchenko, {A. C.} and Miyada, {C. G.} and Goodman, {T. C.} and Walsh, {T. J.} and Brian Wong and Becker, {M. J.} and Ullman, {E. F.}",
year = "1994",
language = "English (US)",
volume = "32",
pages = "92--97",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - An automated enzymatic method for measurement of D-arabinitol, a metabolite of pathogenic Candida species

AU - Switchenko, A. C.

AU - Miyada, C. G.

AU - Goodman, T. C.

AU - Walsh, T. J.

AU - Wong, Brian

AU - Becker, M. J.

AU - Ullman, E. F.

PY - 1994

Y1 - 1994

N2 - An automated enzymatic method was developed for the measurement of D- arabinitol in human serum. The assay is based on a novel, highly specific D- arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH produced is used in a second reaction to reduce p- iodonitrotetrazolium violet (INT) to INT-formazan, which is measured spectrophotometrically. The entire reaction sequence can be performed automatically on a COBAS MIRA-S clinical chemistry analyzer (Roche Diagnostic Systems, Inc., Montclair, N.J.). Replicate analyses of human sera supplemented with D-arabinitol over a concentration range of 0 to 40 μM demonstrated that the pentitol could be measured with an accuracy of ±7% and a precision (standard deviation) of ±0.4 μM. Serum D-arabinitol measurements correlated with those determined by gas chromatography (r = 0.94). The enzymatic method is unaffected by L-arabinitol, D-mannitol, or other polyols commonly found in human serum. Any of 17 therapeutic drugs potentially present in serum did not significantly influence assay performance. Data illustrating the application of the assay in patients for possible diagnosis of invasive candidiasis and the monitoring of therapeutic intervention are presented. The automated assay described here was developed to facilitate the investigation of D-arabinitol as a serum marker for invasive Candida infections.

AB - An automated enzymatic method was developed for the measurement of D- arabinitol in human serum. The assay is based on a novel, highly specific D- arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH produced is used in a second reaction to reduce p- iodonitrotetrazolium violet (INT) to INT-formazan, which is measured spectrophotometrically. The entire reaction sequence can be performed automatically on a COBAS MIRA-S clinical chemistry analyzer (Roche Diagnostic Systems, Inc., Montclair, N.J.). Replicate analyses of human sera supplemented with D-arabinitol over a concentration range of 0 to 40 μM demonstrated that the pentitol could be measured with an accuracy of ±7% and a precision (standard deviation) of ±0.4 μM. Serum D-arabinitol measurements correlated with those determined by gas chromatography (r = 0.94). The enzymatic method is unaffected by L-arabinitol, D-mannitol, or other polyols commonly found in human serum. Any of 17 therapeutic drugs potentially present in serum did not significantly influence assay performance. Data illustrating the application of the assay in patients for possible diagnosis of invasive candidiasis and the monitoring of therapeutic intervention are presented. The automated assay described here was developed to facilitate the investigation of D-arabinitol as a serum marker for invasive Candida infections.

UR - http://www.scopus.com/inward/record.url?scp=0028012398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028012398&partnerID=8YFLogxK

M3 - Article

C2 - 8126210

AN - SCOPUS:0028012398

VL - 32

SP - 92

EP - 97

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 1

ER -