An Arabidopsis thaliana cDNA encoding PS II-X, a 4.1 kDa component of photosystem II: A bipartite presequence mediates SecA/ΔpH-independent targeting into thylakoids

Soo Jung Kim, David Robinson, Colin Robinson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Higher plant photosystem II preparations contain a 4.1 kDa polypeptide (subunit X) associated with the oxygen-evolving core complex. We describe the isolation of a cDNA encoding PS II-X from Arabidopsis thaliana, in which the C-terminal region is highly homologous to partially sequenced PS II-X from wheat and spinach. The mature protein of 42 residues is preceded by a 74-residue, bipartite presequence similar to those involved in the targeting of nuclear-encoded thylakoid lumen proteins, although hydrophobicity analysis indicates the presence of a single transmembrane span in the mature protein. Moreover, import of pre-PS II-X into the thylakoid membrane of isolated chloroplasts is unaffected by inhibitors of either the Sec- or ΔpH-dependent thylakoidal protein translocases, suggesting a spontaneous insertion mechanism. PS II-X appears to be encoded as a mature protein by the plastid genome in the chlorophyll a+c-containing alga, Odontella sinensis. We thus propose that the thylakoid transfer signal of Arabidopsis pre-PS II-X represents a recent acquisition, in phylogenetic terms, compared with signals of Sec-dependent lumenal proteins.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalFEBS Letters
Volume390
Issue number2
DOIs
StatePublished - Jul 22 1996
Externally publishedYes

Fingerprint

Thylakoids
Photosystem II Protein Complex
Arabidopsis
Complementary DNA
Proteins
Chloroplast Proteins
Plastid Genomes
Hydrophobicity
Algae
Spinacia oleracea
Chloroplasts
Hydrophobic and Hydrophilic Interactions
Genes
Triticum
Oxygen
Membranes
Peptides

Keywords

  • Chloroplast
  • Photosynthesis
  • Photosystem II
  • Protein translocation
  • psbX
  • Thylakoid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

An Arabidopsis thaliana cDNA encoding PS II-X, a 4.1 kDa component of photosystem II : A bipartite presequence mediates SecA/ΔpH-independent targeting into thylakoids. / Kim, Soo Jung; Robinson, David; Robinson, Colin.

In: FEBS Letters, Vol. 390, No. 2, 22.07.1996, p. 175-178.

Research output: Contribution to journalArticle

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