An approach toward characterizing the fraction of all oxidation events that attack a particular site within cells during PDT

Steven Jacques, P. Bargo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper considers the the fraction PDT-induced oxidizing radicals that react with a specific oxidizable target within a cell rather than with all possible oxidizable sites. There are many oxidizable sites within the cell, each with a different efficiency of oxidation (Y_ox_j) and a different in vivo concentration (C_iv_j). One measures the efficiency of oxidation of a single ith chemical species in vitro (Y_it_i), then measures the oxidation of the same species in vivo (Y_iv_i). The concentration of this ith species in vivo must be measured (C_iv_i). A convenient test chemical species is chosen, such as a photobleachable fluorophore. Then the in vivo yield is approximately: Y_iv_i = (C_iv_i * Y_it_i) / sum_all_j(C_iv_j * Y_iv_j) (Eq.1). Rearranging to solve for the total oxidation: sum_all_j(C_iv_j * Y_iv_j) = (C_iv_i * Y_it_i) Y_iv_I (Eq.2). Once the sum_all_j() in Eq. 2 is specified, one can measure the in vitro oxidation efficiency and the in vivo concentration of any ith species and use Eq. 1 to predict the fraction of PDT-generated singlet oxygen that will attack that ith species in vivo. Of course, the above is only a first approximation toward a complex problem but is a beginning. This paper illustrates the experimental specification of the Y_ox_j for NADPH oxidation in a cuvette using the photosensitizer Photofrin.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.D. Duncan, S.L. Jacques, P.C. Johnson
Pages65-75
Number of pages11
Volume4257
DOIs
StatePublished - 2001
EventLaser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical - San Jose, CA, United States
Duration: Jan 21 2001Jan 24 2001

Other

OtherLaser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical
CountryUnited States
CitySan Jose, CA
Period1/21/011/24/01

Fingerprint

attack
Oxidation
oxidation
cells
chemical tests
Photosensitizers
Fluorophores
specifications
Specifications
Oxygen
oxygen
approximation

Keywords

  • NADPH
  • Oxidation
  • Photochemistry
  • Photodynamic therapy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Jacques, S., & Bargo, P. (2001). An approach toward characterizing the fraction of all oxidation events that attack a particular site within cells during PDT. In D. D. Duncan, S. L. Jacques, & P. C. Johnson (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4257, pp. 65-75) https://doi.org/10.1117/12.434688

An approach toward characterizing the fraction of all oxidation events that attack a particular site within cells during PDT. / Jacques, Steven; Bargo, P.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D.D. Duncan; S.L. Jacques; P.C. Johnson. Vol. 4257 2001. p. 65-75.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Jacques, S & Bargo, P 2001, An approach toward characterizing the fraction of all oxidation events that attack a particular site within cells during PDT. in DD Duncan, SL Jacques & PC Johnson (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4257, pp. 65-75, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, San Jose, CA, United States, 1/21/01. https://doi.org/10.1117/12.434688
Jacques S, Bargo P. An approach toward characterizing the fraction of all oxidation events that attack a particular site within cells during PDT. In Duncan DD, Jacques SL, Johnson PC, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4257. 2001. p. 65-75 https://doi.org/10.1117/12.434688
Jacques, Steven ; Bargo, P. / An approach toward characterizing the fraction of all oxidation events that attack a particular site within cells during PDT. Proceedings of SPIE - The International Society for Optical Engineering. editor / D.D. Duncan ; S.L. Jacques ; P.C. Johnson. Vol. 4257 2001. pp. 65-75
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