TY - JOUR
T1 - An antigen processing polymorphism revealed by HLA‐B8‐restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism
AU - Rowland‐Jones, Sarah L.
AU - Powis, Stephen H.
AU - Sutton, Julian
AU - Mockridge, Ian
AU - Gotch, Frances M.
AU - Murray, Nick
AU - Hill, Ann B.
AU - Rosenberg, William M.
AU - Trowsdale, John
AU - McMichael, Andrew J.
PY - 1993/8
Y1 - 1993/8
N2 - In previous studies of antigen presentation through HLA‐B27, we identified a healthy person whose lymphoblastoid cells do not present three B27‐restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA‐B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA‐A3‐B2702 haplotype. The original donor, NW, carries HLA‐B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA‐B8‐restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3‐B2702 haplotype, present the expected epitope after viral infection. Another B8‐restricted epitope, from human immunodeficiency virus‐gag, is presented equally well by both cell lines when infected with gag‐vaccinia. This antigen processing phenotype does not correlate with any of the known human TAP‐1 and TAP‐2 polymorphisms.
AB - In previous studies of antigen presentation through HLA‐B27, we identified a healthy person whose lymphoblastoid cells do not present three B27‐restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA‐B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA‐A3‐B2702 haplotype. The original donor, NW, carries HLA‐B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA‐B8‐restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3‐B2702 haplotype, present the expected epitope after viral infection. Another B8‐restricted epitope, from human immunodeficiency virus‐gag, is presented equally well by both cell lines when infected with gag‐vaccinia. This antigen processing phenotype does not correlate with any of the known human TAP‐1 and TAP‐2 polymorphisms.
KW - Antigen processing and presentation
KW - HLA‐B8
KW - TAP‐1 and TAP‐2 genes
UR - http://www.scopus.com/inward/record.url?scp=0027170212&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027170212&partnerID=8YFLogxK
U2 - 10.1002/eji.1830230841
DO - 10.1002/eji.1830230841
M3 - Article
C2 - 8344364
AN - SCOPUS:0027170212
SN - 0014-2980
VL - 23
SP - 1999
EP - 2004
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 8
ER -