An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism

Sarah L. Rowland-Jones, Stephen H. Powis, Julian Sutton, Ian Mockridge, Frances M. Gotch, Nick Murray, Ann Hill, William M. Rosenberg, John Trowsdale, Andrew J. McMichael

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In previous studies of antigen presentation through HLA-B27, we identified a healthy person whose lymphoblastoid cells do not present three B2-restricted viral epitopes to specific cytotoxic T lymphocytes (CTL), despite adequate cell surface expression of HLA-B2702 of normal sequence. Similar findings were observed in all members of his family sharing the HLA-A3-B2702 haplotype. The original donor, NW, carries HLA-B8 on his other class I haplotype, which his daughter, HW, has inherited. We now report a failure to present an HLA-B8-restricted epitope from influenza nucleoprotein following viral infection of NW cells, although exogenous added peptide is still presented normally. However, cells from HW, which do not carry the A3-B2702 haplotype, present the expected epitope after viral infection. Another B8-restricted epitope, from human immunodeficiency virus-gag, is presented eqully well by both cell lines when infected with gag-vaccinia. This amiga processing phenotype does not correlate with any of the known human TAP-1 and TAP-2 polymorphisms.

Original languageEnglish (US)
Pages (from-to)1999-2004
Number of pages6
JournalEuropean Journal of Immunology
Volume23
Issue number8
StatePublished - Aug 1993
Externally publishedYes

Fingerprint

HLA-B8 Antigen
Antigen Presentation
Cytotoxic T-Lymphocytes
Epitopes
Haplotypes
Virus Diseases
Genes
HLA-A3 Antigen
Vaccinia
HLA-B27 Antigen
Nucleoproteins
Nuclear Family
Human Influenza
HIV
Phenotype
Cell Line
Peptides

Keywords

  • Antigen processing and presentation
  • HLA-B8
  • TAP-1 and TAP-2 genes

ASJC Scopus subject areas

  • Immunology

Cite this

Rowland-Jones, S. L., Powis, S. H., Sutton, J., Mockridge, I., Gotch, F. M., Murray, N., ... McMichael, A. J. (1993). An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism. European Journal of Immunology, 23(8), 1999-2004.

An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism. / Rowland-Jones, Sarah L.; Powis, Stephen H.; Sutton, Julian; Mockridge, Ian; Gotch, Frances M.; Murray, Nick; Hill, Ann; Rosenberg, William M.; Trowsdale, John; McMichael, Andrew J.

In: European Journal of Immunology, Vol. 23, No. 8, 08.1993, p. 1999-2004.

Research output: Contribution to journalArticle

Rowland-Jones, SL, Powis, SH, Sutton, J, Mockridge, I, Gotch, FM, Murray, N, Hill, A, Rosenberg, WM, Trowsdale, J & McMichael, AJ 1993, 'An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism', European Journal of Immunology, vol. 23, no. 8, pp. 1999-2004.
Rowland-Jones, Sarah L. ; Powis, Stephen H. ; Sutton, Julian ; Mockridge, Ian ; Gotch, Frances M. ; Murray, Nick ; Hill, Ann ; Rosenberg, William M. ; Trowsdale, John ; McMichael, Andrew J. / An antigen processing polymorphism revealed by HLA-B8-restricted cytotoxic T lymphocytes which does not correlate with TAP gene polymorphism. In: European Journal of Immunology. 1993 ; Vol. 23, No. 8. pp. 1999-2004.
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