An anti-tumor role for cGMP-dependent protein kinase

Yali Hou, Naren Gupta, Patricia Schoenlein, Elsie Wong, Robert Martindale, Vadivel Ganapathy, Darren Browning

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

This study compared Type-1 cGMP-dependent protein kinase (PKG) expression in normal and tumor tissues and examined PKG function in tumor growth. Studies with a cDNA array revealed that PKG expression was reduced in many tumors compared to respective normal tissue. This decrease in PKG expression was confirmed using quantitative RT-PCR and western blotting of matched colon specimens from normal epithelium and tumor tissue, and also in colon derived cell lines where luciferase reporter analysis revealed that the decreased expression occurred at the transcriptional level. Using SW620 colon carcinoma cells engineered for inducible expression of PKG1β, it was found that exogenous PKG1β lead to decreased tumor growth and invasiveness in nude mouse xenografts.

Original languageEnglish (US)
Pages (from-to)60-68
Number of pages9
JournalCancer Letters
Volume240
Issue number1
DOIs
StatePublished - Aug 18 2006

Keywords

  • Colon cancer
  • Gene expression
  • Nude mouse
  • Protein kinase
  • Tumor
  • cGMP

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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