An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C

Andreas E. Busch, Michael D. Varnum, R. Alan North, John Adelman

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

A slowly activating, voltage-dependent potassium channel protein cloned from rat kidney was expressed in Xenopus oocytes. Two activators of protein kinase C, 1-oleoyl-2-acetyl-rac-glycerol and phorbol 12,13-didecanoate, inhibited the current. This inhibition was blocked by the kinase inhibitor staurosporine. Inhibition of the current was not seen in channels in which Ser103 was replaced by Ala, although other properties of the current were unchanged. These results indicate that inhibition of the potassium current results from direct phosphorylation of the channel subunit protein at Ser103.

Original languageEnglish (US)
Pages (from-to)1705-1707
Number of pages3
JournalScience
Volume255
Issue number5052
StatePublished - Mar 27 1992

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Staurosporine
Potassium Channels
Protein Subunits
Xenopus
Protein Kinase C
Oocytes
Potassium
Phosphotransferases
Phosphorylation
Kidney
Amino Acids
Mutation
Proteins
1-oleoyl-2-acetylglycerol
phorbol-12,13-didecanoate

ASJC Scopus subject areas

  • General

Cite this

Busch, A. E., Varnum, M. D., North, R. A., & Adelman, J. (1992). An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C. Science, 255(5052), 1705-1707.

An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C. / Busch, Andreas E.; Varnum, Michael D.; North, R. Alan; Adelman, John.

In: Science, Vol. 255, No. 5052, 27.03.1992, p. 1705-1707.

Research output: Contribution to journalArticle

Busch, AE, Varnum, MD, North, RA & Adelman, J 1992, 'An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C', Science, vol. 255, no. 5052, pp. 1705-1707.
Busch AE, Varnum MD, North RA, Adelman J. An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C. Science. 1992 Mar 27;255(5052):1705-1707.
Busch, Andreas E. ; Varnum, Michael D. ; North, R. Alan ; Adelman, John. / An amino acid mutation in a potassium channel that prevents inhibition by protein kinase C. In: Science. 1992 ; Vol. 255, No. 5052. pp. 1705-1707.
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