An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP

Gary A. Wayman, Monika Davare, Hideaki Ando, Dale Fortin, Olga Varlamova, Hai Ying M Cheng, Daniel Marks, Karl Obrietan, Thomas Soderling, Richard Goodman, Soren Impey

Research output: Contribution to journalArticle

410 Citations (Scopus)

Abstract

Activity-regulated gene expression is believed to play a key role in the development and refinement of neuronal circuitry. Nevertheless, the transcriptional networks that regulate synapse growth and plasticity remain largely uncharacterized. Here, we show that microRNA 132 (miR132) is an activity-dependent rapid response gene regulated by the cAMP response element-binding (CREB) protein pathway. Introduction of miR132 into hippocampal neurons enhanced dendrite morphogenesis whereas inhibition of miR132 by 2′O-methyl RNA antagonists blocked these effects. Furthermore, neuronal activity inhibited translation of p250GAP, a miR132 target, and siRNA-mediated knockdown of p250GAP mimicked miR132-induced dendrite growth. Experiments using dominant-interfering mutants suggested that Rac signaling is downstream of miR132 and p250GAP. We propose that the miR132-p250GAP pathway plays a key role in activity-dependent structural and functional plasticity.

Original languageEnglish (US)
Pages (from-to)9093-9098
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number26
DOIs
StatePublished - Jul 1 2008

Fingerprint

MicroRNAs
Dendrites
Cyclic AMP Response Element-Binding Protein
Gene Regulatory Networks
Growth
Morphogenesis
Synapses
Small Interfering RNA
RNA
Gene Expression
Neurons
Genes

Keywords

  • Actin cytoskeleton
  • CaM kinase
  • cAMP response element-binding (CREB) protein
  • Rac
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP. / Wayman, Gary A.; Davare, Monika; Ando, Hideaki; Fortin, Dale; Varlamova, Olga; Cheng, Hai Ying M; Marks, Daniel; Obrietan, Karl; Soderling, Thomas; Goodman, Richard; Impey, Soren.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 26, 01.07.2008, p. 9093-9098.

Research output: Contribution to journalArticle

Wayman, Gary A. ; Davare, Monika ; Ando, Hideaki ; Fortin, Dale ; Varlamova, Olga ; Cheng, Hai Ying M ; Marks, Daniel ; Obrietan, Karl ; Soderling, Thomas ; Goodman, Richard ; Impey, Soren. / An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 26. pp. 9093-9098.
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AU - Cheng, Hai Ying M

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