An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers

Rajaram Krishnan, David Charlot, Lucas Kumosa, William Hanna, Jerry Lu, Avery Sonnenberg, Michael Heller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.

Original languageEnglish (US)
Title of host publication2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
Pages373-376
Number of pages4
DOIs
StatePublished - 2011
Event2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 - San Diego, CA, United States
Duration: Nov 10 2011Nov 12 2011

Publication series

Name2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011

Conference

Conference2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
CountryUnited States
CitySan Diego, CA
Period11/10/1111/12/11

ASJC Scopus subject areas

  • Hardware and Architecture
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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    Krishnan, R., Charlot, D., Kumosa, L., Hanna, W., Lu, J., Sonnenberg, A., & Heller, M. (2011). An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers. In 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011 (pp. 373-376). [6107805] (2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011). https://doi.org/10.1109/BioCAS.2011.6107805