TY - GEN
T1 - An AC Electrokinetic Device for the rapid separation and detection of cancer related DNA nanoparticulate biomarkers
AU - Krishnan, Rajaram
AU - Charlot, David
AU - Kumosa, Lucas
AU - Hanna, William
AU - Lu, Jerry
AU - Sonnenberg, Avery
AU - Heller, Michael
PY - 2011
Y1 - 2011
N2 - The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.
AB - The ability to rapidly detect cell free circulating (cfc) DNA and other nanoparticulate disease biomarkers directly in blood is a major challenge for nanomedicine. We now show that AC Electrokinetic (ACE) microelectrode array devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates directly from whole blood and other high conductance samples (plasma, serum etc.). The device shows isolation of naked DNA in water and plasma at >500bp size and can isolate DNA down to 50 picograms in 50uL of fluid. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia.
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U2 - 10.1109/BioCAS.2011.6107805
DO - 10.1109/BioCAS.2011.6107805
M3 - Conference contribution
AN - SCOPUS:84862951501
SN - 9781457714696
T3 - 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
SP - 373
EP - 376
BT - 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
T2 - 2011 IEEE Biomedical Circuits and Systems Conference, BioCAS 2011
Y2 - 10 November 2011 through 12 November 2011
ER -