Amyloid precursor proteins interact with the heterotrimeric G protein go in the control of neuronal migration

Jenna M. Ramaker, Tracy L. Swanson, Philip Copenhaver

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Amyloid precursor protein (APP) belongs to a family of evolutionarily conserved transmembrane glycoproteins that has been proposed to regulate multiple aspects of cell motility in the nervous system. AlthoughAPPis best known as the source of β-amyloid fragments (Aβ) that accumulate in Alzheimer's disease, perturbations affecting normal APP signaling events may also contribute to disease progression. Previous in vitro studies showed that interactions between APP and the heterotrimeric G protein Goα-regulated Goα activity and Go-dependent apoptotic responses, independent ofAβ. However, evidence for authenticAPP-Gointeractions within the healthy nervous system has been lacking. To address this issue, we have used a combination of in vitro and in vivo strategies to show that endogenously expressed APP family proteins colocalize with Goα in both insect and mammalian nervous systems, including human brain. Using biochemical, pharmacological, and Bimolecular Fluorescence Complementation assays, we have shown that insect APP (APPL) directly interacts with Goα in cell culture and at synaptic terminals within the insect brain, and that this interaction is regulated by Goα activity. We have also adapted a well characterized assay of neuronal migration in the hawkmoth Manduca to show that perturbations affecting APPL and Goα signaling induce the same unique pattern of ectopic, inappropriate growth and migration, analogous to defective migration patterns seen in mice lacking all APP family proteins. These results support the model that APP and its orthologs regulate conserved aspects of neuronal migration and outgrowth in the nervous system by functioning as unconventional Goα-coupled receptors.

Original languageEnglish (US)
Pages (from-to)10165-10181
Number of pages17
JournalJournal of Neuroscience
Volume33
Issue number24
DOIs
StatePublished - 2013

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Gi-Go GTP-Binding Protein alpha Subunits
Heterotrimeric GTP-Binding Proteins
Amyloid beta-Protein Precursor
Nervous System
Amyloid
Insects
Insect Proteins
Manduca
Presynaptic Terminals
Brain
Cell Movement
Disease Progression
Glycoproteins
Alzheimer Disease
Proteins
Cell Culture Techniques
Fluorescence
Pharmacology
Growth

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Amyloid precursor proteins interact with the heterotrimeric G protein go in the control of neuronal migration. / Ramaker, Jenna M.; Swanson, Tracy L.; Copenhaver, Philip.

In: Journal of Neuroscience, Vol. 33, No. 24, 2013, p. 10165-10181.

Research output: Contribution to journalArticle

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