Amphiphysin 2 (Bin1) and T-tubule biogenesis in muscle

Eunkyung Lee, Melissa Marcucci, Laurie Daniell, Marc Pypaert, Ora A. Weisz, Gian Carlo Ochoa, Khashayar Farsad, Markus R. Wenk, Pietro De Camilli

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In striated muscle, the plasma membrane forms tubular invaginations (transverse tubules or T-tubules) that function in depolarization-contraction coupling. Caveolin-3 and amphiphysin were implicated in their biogenesis. Amphiphysin isoforms have a putative role in membrane deformation at endocytic sites. An isoform of amphiphysin 2 concentrated at T-tubules induced tubular plasma membrane invaginations when expressed in nonmuscle cells. This property required exon 10, a phosphoinositide-binding module. In developing myotubes, amphiphysin 2 and caveolin-3 segregated in tubular and vesicular portions of the T-tubule system, respectively. These findings support a role of the bilayer-deforming properties of amphiphysin at T-tubules and, more generally, a physiological role of amphiphysin in membrane deformation.

Original languageEnglish (US)
Pages (from-to)1193-1196
Number of pages4
Issue number5584
StatePublished - Aug 16 2002

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    Lee, E., Marcucci, M., Daniell, L., Pypaert, M., Weisz, O. A., Ochoa, G. C., Farsad, K., Wenk, M. R., & De Camilli, P. (2002). Amphiphysin 2 (Bin1) and T-tubule biogenesis in muscle. Science, 297(5584), 1193-1196.