Amniotic fluid volume and composition in mouse pregnancy

Cecilia Cheung, Robert A. Brace

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). RESULTS: AF volume increased from 18 ± 4 (SE) μL on day 9.5 to a maximum of 147 ± 4 μL on days 15.5 to 16.5 and decreased sharply to 17 ± 3 μL on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations. CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with AF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition.

Original languageEnglish (US)
Pages (from-to)558-562
Number of pages5
JournalJournal of the Society for Gynecologic Investigation
Volume12
Issue number8
DOIs
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Amniotic Fluid
Pregnancy
Osmolar Concentration
Potassium Chloride
Gestational Age
Statistical Factor Analysis
Young Adult
Lactic Acid
Sheep
Analysis of Variance
Fetus
Sodium
Calcium
Glucose

Keywords

  • Amniotic fluid volume
  • Calcium
  • Chloride
  • Glucose
  • Lactate
  • Mouse
  • Osmolality
  • Potassium
  • Sodium

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Amniotic fluid volume and composition in mouse pregnancy. / Cheung, Cecilia; Brace, Robert A.

In: Journal of the Society for Gynecologic Investigation, Vol. 12, No. 8, 12.2005, p. 558-562.

Research output: Contribution to journalArticle

Cheung, Cecilia ; Brace, Robert A. / Amniotic fluid volume and composition in mouse pregnancy. In: Journal of the Society for Gynecologic Investigation. 2005 ; Vol. 12, No. 8. pp. 558-562.
@article{9deea9e910dc4dc9bd19147375c59f9f,
title = "Amniotic fluid volume and composition in mouse pregnancy",
abstract = "OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). RESULTS: AF volume increased from 18 ± 4 (SE) μL on day 9.5 to a maximum of 147 ± 4 μL on days 15.5 to 16.5 and decreased sharply to 17 ± 3 μL on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations. CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with AF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition.",
keywords = "Amniotic fluid volume, Calcium, Chloride, Glucose, Lactate, Mouse, Osmolality, Potassium, Sodium",
author = "Cecilia Cheung and Brace, {Robert A.}",
year = "2005",
month = "12",
doi = "10.1016/j.jsgi.2005.08.008",
language = "English (US)",
volume = "12",
pages = "558--562",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "8",

}

TY - JOUR

T1 - Amniotic fluid volume and composition in mouse pregnancy

AU - Cheung, Cecilia

AU - Brace, Robert A.

PY - 2005/12

Y1 - 2005/12

N2 - OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). RESULTS: AF volume increased from 18 ± 4 (SE) μL on day 9.5 to a maximum of 147 ± 4 μL on days 15.5 to 16.5 and decreased sharply to 17 ± 3 μL on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations. CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with AF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition.

AB - OBJECTIVE: The current study was undertaken to determine simultaneous changes in amniotic fluid (AF) volume and composition across gestation in the pregnant mouse. METHODS: Young adult mice (6 to 7 weeks old) of the CB6F1 strain were mated overnight. AF was collected on consecutive days from embryonic days 9.5 through 18.5 for measurements of volume and composition. Statistical analysis included one-factor analysis of variance (ANOVA). RESULTS: AF volume increased from 18 ± 4 (SE) μL on day 9.5 to a maximum of 147 ± 4 μL on days 15.5 to 16.5 and decreased sharply to 17 ± 3 μL on day 18.5. AF osmolality was unchanged except for a rise prior to delivery on day 19.5 to 20.5. AF sodium, calcium, and glucose concentrations increased and subsequently decreased as gestation progressed. AF potassium, chloride, and lactate concentrations initially decreased and then increased across gestation. Prior to day 9.5 and after day 18.5, AF volume was too small for volume or compositional determinations. CONCLUSIONS: In the mouse, the rise in AF volume from mid gestation to a maximum late in gestation is similar to that in humans while the sharp fall prior to delivery is not. As observed in the fetal sheep, the changes in fluid volume are associated with AF osmolality and solute concentration changes that are correlated with advancing gestational age. These observations together with the feasibility of quantifying AF volume and composition in the mouse fetus demonstrate the possibility of using genetically altered mice as a model for future studies on the molecular mechanisms underlying the regulation of AF volume and composition.

KW - Amniotic fluid volume

KW - Calcium

KW - Chloride

KW - Glucose

KW - Lactate

KW - Mouse

KW - Osmolality

KW - Potassium

KW - Sodium

UR - http://www.scopus.com/inward/record.url?scp=28444433822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28444433822&partnerID=8YFLogxK

U2 - 10.1016/j.jsgi.2005.08.008

DO - 10.1016/j.jsgi.2005.08.008

M3 - Article

C2 - 16325744

AN - SCOPUS:28444433822

VL - 12

SP - 558

EP - 562

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 8

ER -