Alternative splicing produces messenger rnas encoding insulinlike growth factor-1 prohormones that are differentially glycosylated in vitro

Mark A. Bach, Charles T. Roberts, Eric P. Smith, Derek Leroith

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Rat insulin-like growth factor-1 (IGF-I) cDNA sequences predict two prohormones that differ in the carboxy-terminal extension peptide (E-peptide) as a result of the inclusion or exclusion of the 52-basepair exon 4 sequence. In the absence of exon 4, the sequence codes for the IGF-la prohormone, whose E region contains two potential N-glycosylation sites. With differential splicing and the inclusion of exon 4, the resultant mRNA codes for IGF-lb, with a longer E-region sequence. In addition, as a consequence of a frame shift, both potential glycosylation sites are lost in the IGF-lb peptide. We used an in vitro translation system supplemented with canine pancreatic microsomal membranes to analyze cotranslational processing of the IGF-I propeptides. We have demonstrated that the IGF-la prohormone, which contains two potential N-glycosylation sites in the E region, can be N-glycosylated in vitro, and that both glycosylation sites are probably used. As expected, the IGF-Ib preprohormone is processed by microsomes, but is not glycosylated.

Original languageEnglish (US)
Pages (from-to)899-904
Number of pages6
JournalMolecular Endocrinology
Volume4
Issue number6
DOIs
StatePublished - Jun 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Fingerprint

Dive into the research topics of 'Alternative splicing produces messenger rnas encoding insulinlike growth factor-1 prohormones that are differentially glycosylated in vitro'. Together they form a unique fingerprint.

Cite this