Alternate pathways of DNA replication

DNA polymerase I-dependent replication

O. Niwa, S. K. Bryan, Robb Moses

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have previously shown that some Escherichia coli (derivatives of strain HS432 (polA1, polB100, polC1026) can replicate DNA at a restrictive temperature in the presence of a polCts mutation and that such revertants contain apparent DNA polymerase I activity. We demonstrate here that this strain of E. coli becomes temperature-resistant upon the introduction of a normal gene for DNA polymerase I or suppression of the polA1 nonsense mutation. Such temperature-resistant phenocopies become temperature-sensitive upon introduction of a temperature-sensitive DNA polymerase I gene. Our results confirm that DNA replication is DNA polymerase I-dependent in the temperature resistant revertants, indicating that an alternative pathway of replication exists in E. coli. HS432 contains a transducible locus (which we term pcbA) that can support an alternate pathway in other E. coli strains, so the effect of suppression of polCts is a general one.

Original languageEnglish (US)
Pages (from-to)7024-7027
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number11 II
StatePublished - 1981
Externally publishedYes

Fingerprint

DNA Polymerase I
DNA Replication
Temperature
Escherichia coli
Nonsense Codon
Genes
Mutation
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Alternate pathways of DNA replication : DNA polymerase I-dependent replication. / Niwa, O.; Bryan, S. K.; Moses, Robb.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, No. 11 II, 1981, p. 7024-7027.

Research output: Contribution to journalArticle

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