TY - JOUR
T1 - Alternate pathways of DNA replication
T2 - DNA polymerase I-dependent replication
AU - Niwa, O.
AU - Bryan, S. K.
AU - Moses, R. E.
PY - 1981
Y1 - 1981
N2 - We have previously shown that some Escherichia coli (derivatives of strain HS432 (polA1, polB100, polC1026) can replicate DNA at a restrictive temperature in the presence of a polCts mutation and that such revertants contain apparent DNA polymerase I activity. We demonstrate here that this strain of E. coli becomes temperature-resistant upon the introduction of a normal gene for DNA polymerase I or suppression of the polA1 nonsense mutation. Such temperature-resistant phenocopies become temperature-sensitive upon introduction of a temperature-sensitive DNA polymerase I gene. Our results confirm that DNA replication is DNA polymerase I-dependent in the temperature resistant revertants, indicating that an alternative pathway of replication exists in E. coli. HS432 contains a transducible locus (which we term pcbA) that can support an alternate pathway in other E. coli strains, so the effect of suppression of polCts is a general one.
AB - We have previously shown that some Escherichia coli (derivatives of strain HS432 (polA1, polB100, polC1026) can replicate DNA at a restrictive temperature in the presence of a polCts mutation and that such revertants contain apparent DNA polymerase I activity. We demonstrate here that this strain of E. coli becomes temperature-resistant upon the introduction of a normal gene for DNA polymerase I or suppression of the polA1 nonsense mutation. Such temperature-resistant phenocopies become temperature-sensitive upon introduction of a temperature-sensitive DNA polymerase I gene. Our results confirm that DNA replication is DNA polymerase I-dependent in the temperature resistant revertants, indicating that an alternative pathway of replication exists in E. coli. HS432 contains a transducible locus (which we term pcbA) that can support an alternate pathway in other E. coli strains, so the effect of suppression of polCts is a general one.
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U2 - 10.1073/pnas.78.11.7024
DO - 10.1073/pnas.78.11.7024
M3 - Article
C2 - 7031666
AN - SCOPUS:0019860892
SN - 0027-8424
VL - 78
SP - 7024
EP - 7027
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11 II
ER -