Altered vertebral and femoral bone structure in juvenile offspring of microswine subject to maternal low protein nutritional challenge

Stuart A. Lanham, Elizabeth DuPriest, Philipp Kupfer, Cyrus Cooper, Susan Bagby, Richard O.C. Oreffo

Research output: Contribution to journalArticle

Abstract

Epidemiological studies suggest skeletal growth is programmed during intrauterine and early postnatal life. We hypothesize that bone development may be altered by maternal diet and have investigated this using a microswine model of maternal protein restriction (MPR). Mothers were fed a control diet (14% protein) or isocaloric low (1%) protein diet during late pregnancy and for 2 weeks postnatally. Offspring were weaned at 4 weeks of age to ad lib or calorie-restricted food intake groups. Femur and vertebra were analysed by micro computed tomography in offspring 3–5 months of age. Caloric restriction from 4 weeks of age, designed to prevent catch-up growth, showed no significant effects on bone structure in the offspring from either maternal dietary group. A maternal low protein diet altered trabecular number in the proximal femur and vertebra in juvenile offspring. Cortical bone was unaffected. These results further support the need to understand the key role of the nutritional environment in early development on programming of skeletal development and consequences in later life.

Original languageEnglish (US)
Article numbere14081
JournalPhysiological Reports
Volume7
Issue number11
DOIs
StatePublished - Jun 1 2019

Fingerprint

Thigh
Mothers
Bone and Bones
Femur
Proteins
Spine
Diet
Caloric Restriction
Protein-Restricted Diet
Bone Development
Growth
Epidemiologic Studies
Eating
Tomography
Pregnancy

Keywords

  • Bone
  • computed tomography
  • intrauterine programming
  • low protein

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Altered vertebral and femoral bone structure in juvenile offspring of microswine subject to maternal low protein nutritional challenge. / Lanham, Stuart A.; DuPriest, Elizabeth; Kupfer, Philipp; Cooper, Cyrus; Bagby, Susan; Oreffo, Richard O.C.

In: Physiological Reports, Vol. 7, No. 11, e14081, 01.06.2019.

Research output: Contribution to journalArticle

Lanham, Stuart A. ; DuPriest, Elizabeth ; Kupfer, Philipp ; Cooper, Cyrus ; Bagby, Susan ; Oreffo, Richard O.C. / Altered vertebral and femoral bone structure in juvenile offspring of microswine subject to maternal low protein nutritional challenge. In: Physiological Reports. 2019 ; Vol. 7, No. 11.
@article{038e991a701c4de6aebf90c1e03b49ab,
title = "Altered vertebral and femoral bone structure in juvenile offspring of microswine subject to maternal low protein nutritional challenge",
abstract = "Epidemiological studies suggest skeletal growth is programmed during intrauterine and early postnatal life. We hypothesize that bone development may be altered by maternal diet and have investigated this using a microswine model of maternal protein restriction (MPR). Mothers were fed a control diet (14{\%} protein) or isocaloric low (1{\%}) protein diet during late pregnancy and for 2 weeks postnatally. Offspring were weaned at 4 weeks of age to ad lib or calorie-restricted food intake groups. Femur and vertebra were analysed by micro computed tomography in offspring 3–5 months of age. Caloric restriction from 4 weeks of age, designed to prevent catch-up growth, showed no significant effects on bone structure in the offspring from either maternal dietary group. A maternal low protein diet altered trabecular number in the proximal femur and vertebra in juvenile offspring. Cortical bone was unaffected. These results further support the need to understand the key role of the nutritional environment in early development on programming of skeletal development and consequences in later life.",
keywords = "Bone, computed tomography, intrauterine programming, low protein",
author = "Lanham, {Stuart A.} and Elizabeth DuPriest and Philipp Kupfer and Cyrus Cooper and Susan Bagby and Oreffo, {Richard O.C.}",
year = "2019",
month = "6",
day = "1",
doi = "10.14814/phy2.14081",
language = "English (US)",
volume = "7",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Altered vertebral and femoral bone structure in juvenile offspring of microswine subject to maternal low protein nutritional challenge

AU - Lanham, Stuart A.

AU - DuPriest, Elizabeth

AU - Kupfer, Philipp

AU - Cooper, Cyrus

AU - Bagby, Susan

AU - Oreffo, Richard O.C.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Epidemiological studies suggest skeletal growth is programmed during intrauterine and early postnatal life. We hypothesize that bone development may be altered by maternal diet and have investigated this using a microswine model of maternal protein restriction (MPR). Mothers were fed a control diet (14% protein) or isocaloric low (1%) protein diet during late pregnancy and for 2 weeks postnatally. Offspring were weaned at 4 weeks of age to ad lib or calorie-restricted food intake groups. Femur and vertebra were analysed by micro computed tomography in offspring 3–5 months of age. Caloric restriction from 4 weeks of age, designed to prevent catch-up growth, showed no significant effects on bone structure in the offspring from either maternal dietary group. A maternal low protein diet altered trabecular number in the proximal femur and vertebra in juvenile offspring. Cortical bone was unaffected. These results further support the need to understand the key role of the nutritional environment in early development on programming of skeletal development and consequences in later life.

AB - Epidemiological studies suggest skeletal growth is programmed during intrauterine and early postnatal life. We hypothesize that bone development may be altered by maternal diet and have investigated this using a microswine model of maternal protein restriction (MPR). Mothers were fed a control diet (14% protein) or isocaloric low (1%) protein diet during late pregnancy and for 2 weeks postnatally. Offspring were weaned at 4 weeks of age to ad lib or calorie-restricted food intake groups. Femur and vertebra were analysed by micro computed tomography in offspring 3–5 months of age. Caloric restriction from 4 weeks of age, designed to prevent catch-up growth, showed no significant effects on bone structure in the offspring from either maternal dietary group. A maternal low protein diet altered trabecular number in the proximal femur and vertebra in juvenile offspring. Cortical bone was unaffected. These results further support the need to understand the key role of the nutritional environment in early development on programming of skeletal development and consequences in later life.

KW - Bone

KW - computed tomography

KW - intrauterine programming

KW - low protein

UR - http://www.scopus.com/inward/record.url?scp=85067187469&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067187469&partnerID=8YFLogxK

U2 - 10.14814/phy2.14081

DO - 10.14814/phy2.14081

M3 - Article

VL - 7

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 11

M1 - e14081

ER -