Altered levels of endogenous retrovirus‐like sequence (VL30) RNA during mouse epidermal cell carcinogenesis

Kyung‐An ‐A Han, Paul Rothberg, Molly Kulesz‐Martin

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Gene expression during mouse keratinocyte carcinogenesis was examined in a clonal cell model. Tumor cells from three separate initiated cell lineages were compared with their nontumorigenic precursors and with the progenitor cell strain prior to treatment with 7,12‐dimethylbenzaanthracene (DMBA). The steady‐state levels of VL30 RNA in normal and papilloma cells were regulated by extracellular Ca2+ (which controls proliferation and differentiation in normal epidermal keratinocytes) and culture density. In contrast, steady‐state levels of VL30 RNA were not regulated by these factors in the squamous cell carcinoma or the anaplastic carcinoma cells. VL30 expression was Ca2+ dependent in the initiated cell precursors within each tumor cell lineage, suggesting that the loss of response to extracellular Ca2+ was associated with the malignant conversion stage of carcinogenesis. No differences between normal and tumor cells were found in the cellular RNA levels of five additional proto‐oncogenes. The mouse epidermal cell model should provide a means for direct assessment of a potential functional role of VL30 sequences in cancer development.

Original languageEnglish (US)
Pages (from-to)75-82
Number of pages8
JournalMolecular Carcinogenesis
Volume3
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • Gene expression
  • Key words
  • cell differentiation
  • epithelial carcinogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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