Altered expression of wild-type p53 tumor suppressor gene during murine epithelial cell transformation

Kyung An Han, Molly Kulesz-Martin

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

An epidermal cell model in which initiated, benign tumor-producing and carcinoma stages were derived from a cloned parental cell strain was used to examine p53 expression during multistage epithelial carcinogenesis. Increased steady-state levels of p53 RNA were detected in squamous cell carcinomas compared to papilloma and normal epidermal cells. Nontumorigenic initiated cell precursors of the carcinomas exhibited normal p53 expression, localizing altered p53 regulation to the malignant conversion stage. Immunoprecipitation and Western immunoblot analyses demonstrated elevated levels of p53 protein in the moderately differentiated carcinoma compared to normal cells, and negligible levels of p53 in the poorly differentiated carcinoma cells. Sequence analysis of p53 complementary DNA from normal and carcinoma cells revealed no mutations in the coding or 5′- and 3′-untranslated regions, suggesting a novel mechanism of p53 inactivation.

Original languageEnglish (US)
Pages (from-to)749-753
Number of pages5
JournalCancer Research
Volume52
Issue number3
StatePublished - Feb 1 1992
Externally publishedYes

Fingerprint

Tumor Suppressor Genes
Epithelial Cells
Carcinoma
5' Untranslated Regions
Papilloma
3' Untranslated Regions
Immunoprecipitation
Sequence Analysis
Squamous Cell Carcinoma
Carcinogenesis
Complementary DNA
Western Blotting
RNA
Mutation
Neoplasms
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Altered expression of wild-type p53 tumor suppressor gene during murine epithelial cell transformation. / Han, Kyung An; Kulesz-Martin, Molly.

In: Cancer Research, Vol. 52, No. 3, 01.02.1992, p. 749-753.

Research output: Contribution to journalArticle

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