TY - JOUR
T1 - Altered expression and phosphorylation of N-methyl-D-aspartate receptors in piglet striatum after hypoxia - Ischemia
AU - Guerguerian, Anne Marie
AU - Brambrink, Ansgar M.
AU - Traystman, Richard J.
AU - Huganir, Richard L.
AU - Martin, Lee J.
N1 - Funding Information:
The authors are grateful for the outstanding technical support of Ms. Ann Price and Mr. Frank Barksdale and for the editorial support of Dr. Deborah McLellan. This research was supported by grants from the National Institutes of Health, NS34100 (L.J.M.), AG16282 (L.J.M.) and NS20020 (R.J.T.), and from the US Army Medical Research and Materiel Command (DAMD17-99-1-9553, L.J.M.). Anne-Marie Guerguerian is the recipient of a Young Investigator Award from the World Federation of Pediatric Intensive and Critical Care Societies.
PY - 2002/7/15
Y1 - 2002/7/15
N2 - The mechanisms for the profound degeneration of striatal neurons after hypoxia - ischemia in newborns are not understood. We hypothesized that this striatal neurodegeneration is related to N-methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity. Using a 1-week-old piglet model of hypoxia - ischemia, we evaluated whether the expression and phosphorylation of NMDA receptor subunits in striatum are modified with severity of evolving neuronal injury after hypoxia - ischemia. Protein levels of NR1, phosphorylated NR1 897serine, NR2A and NR2B in striatum were measured by immunoblotting after piglets underwent hypoxic - asphyxic cardiac arrest, cardiopulmonary resuscitation, and recovery for 3, 6, 12 or 24 h. In membrane fractions isolated from total striatum, mean NR1 and NR2A levels did not change significantly with time after hypoxia - ischemia compared to control; however, the levels of both NR1 and phosphorylated NR1 897serine correlated with neuronal injury in putamen, with higher levels associated with greater neuronal injury in individual animals. NR2B levels were increased at 24 h after hypoxia - ischemia. Astrocyte expression of NR2B was prominent after hypoxia - ischemia. We conclude that NMDA receptors are changed in striatum after neonatal hypoxia - ischemia and that abnormal NMDA receptor potentiation through increased NR1 phosphorylation may participate in the mechanisms of striatal neuron degeneration after hypoxia - ischemia.
AB - The mechanisms for the profound degeneration of striatal neurons after hypoxia - ischemia in newborns are not understood. We hypothesized that this striatal neurodegeneration is related to N-methyl-D-aspartate (NMDA) receptor-mediated excitotoxicity. Using a 1-week-old piglet model of hypoxia - ischemia, we evaluated whether the expression and phosphorylation of NMDA receptor subunits in striatum are modified with severity of evolving neuronal injury after hypoxia - ischemia. Protein levels of NR1, phosphorylated NR1 897serine, NR2A and NR2B in striatum were measured by immunoblotting after piglets underwent hypoxic - asphyxic cardiac arrest, cardiopulmonary resuscitation, and recovery for 3, 6, 12 or 24 h. In membrane fractions isolated from total striatum, mean NR1 and NR2A levels did not change significantly with time after hypoxia - ischemia compared to control; however, the levels of both NR1 and phosphorylated NR1 897serine correlated with neuronal injury in putamen, with higher levels associated with greater neuronal injury in individual animals. NR2B levels were increased at 24 h after hypoxia - ischemia. Astrocyte expression of NR2B was prominent after hypoxia - ischemia. We conclude that NMDA receptors are changed in striatum after neonatal hypoxia - ischemia and that abnormal NMDA receptor potentiation through increased NR1 phosphorylation may participate in the mechanisms of striatal neuron degeneration after hypoxia - ischemia.
KW - Excitotoxicity
KW - Neuronal cell death
UR - http://www.scopus.com/inward/record.url?scp=0037099230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037099230&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(02)00285-1
DO - 10.1016/S0169-328X(02)00285-1
M3 - Article
C2 - 12117552
AN - SCOPUS:0037099230
SN - 0169-328X
VL - 104
SP - 66
EP - 80
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -