Alterations in T-cell subset frequency in peripheral blood in obesity

Robert W. O'Rourke, Thomas Kay, Mark H. Scholz, Brian Diggs, Blair A. Jobe, David Lewinsohn, Antony C. Bakke

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Background: Obesity affects the regulation of immune and inflammatory responses. This study characterizes differences in peripheral blood lymphocyte phenotype in obese humans. Methods: Frequencies of lymphocyte subsets among peripheral blood mononuclear cells were compared between 10 obese (BMI ≥35) and 10 lean subjects, as determined by antibodies directed against cluster differentiation (CD) markers. Results: Obese patients demonstrated an increased frequency of CD3+CD4+ T-cells (mean difference 12%, P=0.004), a decreased frequency of CD3+CD8+ T-cells (mean difference 9.4%, P=0.016) and an increased frequency of CD3+CD8+CD95+ T-cells (mean difference 13.3%, P=0.032). No other differences among T-cell or monocyte subsets were noted. Conclusions: Obesity is associated with alterations in frequencies of peripheral CD4+ and CD8+ T-cells and aberrations in the expression of CD95 among CD8+T-cells. These data suggest both CD4+ and CD8+ T-cell compartments, as well as the regulation of CD95 expression on CD8+ T-cells, as targets for further study into obesity's effects on the immune system.

Original languageEnglish (US)
Pages (from-to)1463-1468
Number of pages6
JournalObesity Surgery
Volume15
Issue number10
DOIs
StatePublished - Nov 2005

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T-Lymphocyte Subsets
Obesity
T-Lymphocytes
CD Antigens
Lymphocyte Subsets
Monocytes
Immune System
Blood Cells
Lymphocytes
Phenotype
Antibodies

Keywords

  • Flow cytometry
  • Immunity
  • Lymphocyte
  • Morbid obesity
  • T-cell

ASJC Scopus subject areas

  • Surgery

Cite this

O'Rourke, R. W., Kay, T., Scholz, M. H., Diggs, B., Jobe, B. A., Lewinsohn, D., & Bakke, A. C. (2005). Alterations in T-cell subset frequency in peripheral blood in obesity. Obesity Surgery, 15(10), 1463-1468. https://doi.org/10.1381/096089205774859308

Alterations in T-cell subset frequency in peripheral blood in obesity. / O'Rourke, Robert W.; Kay, Thomas; Scholz, Mark H.; Diggs, Brian; Jobe, Blair A.; Lewinsohn, David; Bakke, Antony C.

In: Obesity Surgery, Vol. 15, No. 10, 11.2005, p. 1463-1468.

Research output: Contribution to journalArticle

O'Rourke, RW, Kay, T, Scholz, MH, Diggs, B, Jobe, BA, Lewinsohn, D & Bakke, AC 2005, 'Alterations in T-cell subset frequency in peripheral blood in obesity', Obesity Surgery, vol. 15, no. 10, pp. 1463-1468. https://doi.org/10.1381/096089205774859308
O'Rourke, Robert W. ; Kay, Thomas ; Scholz, Mark H. ; Diggs, Brian ; Jobe, Blair A. ; Lewinsohn, David ; Bakke, Antony C. / Alterations in T-cell subset frequency in peripheral blood in obesity. In: Obesity Surgery. 2005 ; Vol. 15, No. 10. pp. 1463-1468.
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AB - Background: Obesity affects the regulation of immune and inflammatory responses. This study characterizes differences in peripheral blood lymphocyte phenotype in obese humans. Methods: Frequencies of lymphocyte subsets among peripheral blood mononuclear cells were compared between 10 obese (BMI ≥35) and 10 lean subjects, as determined by antibodies directed against cluster differentiation (CD) markers. Results: Obese patients demonstrated an increased frequency of CD3+CD4+ T-cells (mean difference 12%, P=0.004), a decreased frequency of CD3+CD8+ T-cells (mean difference 9.4%, P=0.016) and an increased frequency of CD3+CD8+CD95+ T-cells (mean difference 13.3%, P=0.032). No other differences among T-cell or monocyte subsets were noted. Conclusions: Obesity is associated with alterations in frequencies of peripheral CD4+ and CD8+ T-cells and aberrations in the expression of CD95 among CD8+T-cells. These data suggest both CD4+ and CD8+ T-cell compartments, as well as the regulation of CD95 expression on CD8+ T-cells, as targets for further study into obesity's effects on the immune system.

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