Alteration of the fatty acid composition of membrane phospholipids in mouse lymphoid cells

Gail Mandel, S. Shimizu, R. Gill, W. Clark

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A simple method is described for introducing exogenous fatty acids into the membrane phospholipids of the murine leukemia cell EL-4, and into the membrane phospholipids of resting mouse lymphocytes. The method involves culturing of the cells with free or methylated fatty acids at concentrations up to 50 μg/ml. The presence of serum in the culture medium does not interfere with fatty acid uptake, but does increase the growth rate and viability of the cells. Membrane lipid composition returns to normal after the cells are grown in medium without exogenous fatty acid. Fractionation of the cell membranes confirmed that exogenous fatty acids were incorporated into the phospholipids of the plasma membrane.

Original languageEnglish (US)
Pages (from-to)1631-1636
Number of pages6
JournalJournal of Immunology
Volume120
Issue number5
StatePublished - 1978
Externally publishedYes

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Phospholipids
Fatty Acids
Lymphocytes
Membranes
Cell Membrane
Membrane Lipids
Culture Media
Cell Survival
Leukemia
Growth
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Alteration of the fatty acid composition of membrane phospholipids in mouse lymphoid cells. / Mandel, Gail; Shimizu, S.; Gill, R.; Clark, W.

In: Journal of Immunology, Vol. 120, No. 5, 1978, p. 1631-1636.

Research output: Contribution to journalArticle

Mandel, Gail ; Shimizu, S. ; Gill, R. ; Clark, W. / Alteration of the fatty acid composition of membrane phospholipids in mouse lymphoid cells. In: Journal of Immunology. 1978 ; Vol. 120, No. 5. pp. 1631-1636.
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