alpha-chain sequence of newt haemoglobin (Taricha granulosa).

M. Coates, B. Brimhall, Peter Stenzel, M. Hermodson, D. Gibson, R. T. Jones, T. Vedvick

Research output: Contribution to journalArticle

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Abstract

The amino acid sequence of the alpha-chain of the major haemoglobin of a newt, T. granulosa, has been determined. The chain is 142 residues long and has an extra methionine at its N-terminus when compared with human alpha-chain. Most of the tryptic peptides were sequenced by a combination of the subtractive Edman method and by deduction from the compositions of overlapping fragments produced by various enzymic treatments. The sequence of two 'core' regions was obtained by automatic sequencing of large peptides produced by trypsin cleavage at arginine residues only after blockage of lysine residues by citraconylation; by cleavage between aspartic acid and proline residues with 70% formic acid, and by cyanogen bromide cleavage at methionine residues. The sequence of T. granulosa alpha-chain is compared with those of representative species from the other classes of vertebrates. The differences in alpha-chain between the classes of vertebrates are compared with the differences in this protein between an equal number of orders of mammals. This comparison allows us to conclude that the major functional and conformational features of alpha-chain have been conserved since the divergence of the classes of jawed vertebrates.

Original languageEnglish (US)
Pages (from-to)1-19
Number of pages19
JournalAustralian Journal of Biological Sciences
Volume30
Issue number1-2
StatePublished - Apr 1977

Fingerprint

Salamandridae
salamanders and newts
Vertebrates
hemoglobin
Hemoglobins
formic acid
vertebrates
Methionine
methionine
peptides
cyanogen
Cyanogen Bromide
Peptides
enzymatic treatment
aspartic acid
bromides
Proline
Aspartic Acid
Trypsin
trypsin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Coates, M., Brimhall, B., Stenzel, P., Hermodson, M., Gibson, D., Jones, R. T., & Vedvick, T. (1977). alpha-chain sequence of newt haemoglobin (Taricha granulosa). Australian Journal of Biological Sciences, 30(1-2), 1-19.

alpha-chain sequence of newt haemoglobin (Taricha granulosa). / Coates, M.; Brimhall, B.; Stenzel, Peter; Hermodson, M.; Gibson, D.; Jones, R. T.; Vedvick, T.

In: Australian Journal of Biological Sciences, Vol. 30, No. 1-2, 04.1977, p. 1-19.

Research output: Contribution to journalArticle

Coates, M, Brimhall, B, Stenzel, P, Hermodson, M, Gibson, D, Jones, RT & Vedvick, T 1977, 'alpha-chain sequence of newt haemoglobin (Taricha granulosa).', Australian Journal of Biological Sciences, vol. 30, no. 1-2, pp. 1-19.
Coates M, Brimhall B, Stenzel P, Hermodson M, Gibson D, Jones RT et al. alpha-chain sequence of newt haemoglobin (Taricha granulosa). Australian Journal of Biological Sciences. 1977 Apr;30(1-2):1-19.
Coates, M. ; Brimhall, B. ; Stenzel, Peter ; Hermodson, M. ; Gibson, D. ; Jones, R. T. ; Vedvick, T. / alpha-chain sequence of newt haemoglobin (Taricha granulosa). In: Australian Journal of Biological Sciences. 1977 ; Vol. 30, No. 1-2. pp. 1-19.
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