Allosteric activation of functionally asymmetric RAF kinase dimers

Jiancheng Hu, Edward C. Stites, Haiyang Yu, Elizabeth A. Germino, Hiruy S. Meharena, Philip Stork, Alexandr P. Kornev, Susan S. Taylor, Andrey S. Shaw

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Although RAF kinases are critical for controlling cell growth, their mechanism of activation is incompletely understood. Recently, dimerization was shown to be important for activation. Here we show that the dimer is functionally asymmetric with one kinase functioning as an activator to stimulate activity of the partner, receiver kinase. The activator kinase did not require kinase activity but did require N-terminal phosphorylation that functioned allosterically to induce cis-autophosphorylation of the receiver kinase. Based on modeling of the hydrophobic spine assembly, we also engineered a constitutively active mutant that was independent of Ras, dimerization, and activation-loop phosphorylation. As N-terminal phosphorylation of BRAF is constitutive, BRAF initially functions to activate CRAF. N-terminal phosphorylation of CRAF was dependent on MEK, suggesting a feedback mechanism and explaining a key difference between BRAF and CRAF. Our work illuminates distinct steps in RAF activation that function to assemble the active conformation of the RAF kinase.

Original languageEnglish (US)
Pages (from-to)1036-1046
Number of pages11
JournalCell
Volume154
Issue number5
DOIs
StatePublished - Aug 29 2013

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Dimers
Phosphotransferases
Chemical activation
Phosphorylation
Dimerization
Mitogen-Activated Protein Kinase Kinases
Cell growth
Conformations
Spine
Feedback
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hu, J., Stites, E. C., Yu, H., Germino, E. A., Meharena, H. S., Stork, P., ... Shaw, A. S. (2013). Allosteric activation of functionally asymmetric RAF kinase dimers. Cell, 154(5), 1036-1046. https://doi.org/10.1016/j.cell.2013.07.046

Allosteric activation of functionally asymmetric RAF kinase dimers. / Hu, Jiancheng; Stites, Edward C.; Yu, Haiyang; Germino, Elizabeth A.; Meharena, Hiruy S.; Stork, Philip; Kornev, Alexandr P.; Taylor, Susan S.; Shaw, Andrey S.

In: Cell, Vol. 154, No. 5, 29.08.2013, p. 1036-1046.

Research output: Contribution to journalArticle

Hu, J, Stites, EC, Yu, H, Germino, EA, Meharena, HS, Stork, P, Kornev, AP, Taylor, SS & Shaw, AS 2013, 'Allosteric activation of functionally asymmetric RAF kinase dimers', Cell, vol. 154, no. 5, pp. 1036-1046. https://doi.org/10.1016/j.cell.2013.07.046
Hu J, Stites EC, Yu H, Germino EA, Meharena HS, Stork P et al. Allosteric activation of functionally asymmetric RAF kinase dimers. Cell. 2013 Aug 29;154(5):1036-1046. https://doi.org/10.1016/j.cell.2013.07.046
Hu, Jiancheng ; Stites, Edward C. ; Yu, Haiyang ; Germino, Elizabeth A. ; Meharena, Hiruy S. ; Stork, Philip ; Kornev, Alexandr P. ; Taylor, Susan S. ; Shaw, Andrey S. / Allosteric activation of functionally asymmetric RAF kinase dimers. In: Cell. 2013 ; Vol. 154, No. 5. pp. 1036-1046.
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