Alleles of the yeast PMS1 mismatch-repair gene that differentially affect recombination- and replication-related processes

Caroline Welz-Voegele, Jana E. Stone, Phuoc T. Tran, Hutton M. Kearney, Robert (Mike) Liskay, Thomas D. Petes, Sue Jinks-Robertson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mismatch-repair (MMR) systems promote eukaryotic genome stability by removing errors introduced during DNA replication and by inhibiting recombination between nonidentical sequences (spellchecker and antirecombination activities, respectively). Following a common mismatch-recognition step effected by MutS-homologous Msh proteins, homologs of the bacterial MutL ATPase (predominantly the Mlh1p-Pms1p heterodimer in yeast) couple mismatch recognition to the appropriate downstream processing steps. To examine whether the processing steps in the spellchecker and antirecombination pathways might differ, we mutagenized the yeast PMS1 gene and screened for mitotic separation-of-function alleles. Two alleles affecting only the antirecombination function of Pms1p were identified, one of which changed an amino acid within the highly conserved ATPase domain. To more specifically address the role of ATP binding/hydrolysis in MMR-related processes, we examined mutations known to compromise the ATPase activity of Pms1p or Mlh1p with respect to the mitotic spellchecker and antirecombination activities and with respect to the repair of mismatches present in meiotic recombination intermediates. The results of these analyses confirm a differential requirement for the Pms1p ATPase activity in replication vs. recombination processes, while demonstrating that the Mlhlp ATPase activity is important for all examined MMR-related functions.

Original languageEnglish (US)
Pages (from-to)1131-1145
Number of pages15
JournalGenetics
Volume162
Issue number3
StatePublished - Nov 1 2002

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DNA Mismatch Repair
Genetic Recombination
Adenosine Triphosphatases
Yeasts
Alleles
Genes
Bacterial Proteins
Genomic Instability
DNA Replication
Hydrolysis
Adenosine Triphosphate
Amino Acids
Mutation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Welz-Voegele, C., Stone, J. E., Tran, P. T., Kearney, H. M., Liskay, R. M., Petes, T. D., & Jinks-Robertson, S. (2002). Alleles of the yeast PMS1 mismatch-repair gene that differentially affect recombination- and replication-related processes. Genetics, 162(3), 1131-1145.

Alleles of the yeast PMS1 mismatch-repair gene that differentially affect recombination- and replication-related processes. / Welz-Voegele, Caroline; Stone, Jana E.; Tran, Phuoc T.; Kearney, Hutton M.; Liskay, Robert (Mike); Petes, Thomas D.; Jinks-Robertson, Sue.

In: Genetics, Vol. 162, No. 3, 01.11.2002, p. 1131-1145.

Research output: Contribution to journalArticle

Welz-Voegele, C, Stone, JE, Tran, PT, Kearney, HM, Liskay, RM, Petes, TD & Jinks-Robertson, S 2002, 'Alleles of the yeast PMS1 mismatch-repair gene that differentially affect recombination- and replication-related processes', Genetics, vol. 162, no. 3, pp. 1131-1145.
Welz-Voegele C, Stone JE, Tran PT, Kearney HM, Liskay RM, Petes TD et al. Alleles of the yeast PMS1 mismatch-repair gene that differentially affect recombination- and replication-related processes. Genetics. 2002 Nov 1;162(3):1131-1145.
Welz-Voegele, Caroline ; Stone, Jana E. ; Tran, Phuoc T. ; Kearney, Hutton M. ; Liskay, Robert (Mike) ; Petes, Thomas D. ; Jinks-Robertson, Sue. / Alleles of the yeast PMS1 mismatch-repair gene that differentially affect recombination- and replication-related processes. In: Genetics. 2002 ; Vol. 162, No. 3. pp. 1131-1145.
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