Alignment of the transcriptome with individual variation in animals selectively bred for High Drinking-In-the-Dark (HDID)

Robert Hitzemann, Denesa Oberbeck, Ovidiu Iancu, Priscila Darakjian, Shannon McWeeney, Stephanie Spence, Jason Schlumbohm, Pamela Metten, John Jr Crabbe

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Among animals at risk for excessive ethanol consumption such as the HDID selected mouse lines, there is considerable individual variation in the amount of ethanol consumed and the associated blood ethanol concentrations (BECs). For the HDID lines, this variation occurs even though the residual genetic variation associated with the DID phenotype has been largely exhausted and thus is most likely associated with epigenetic factors. Here we focus on the question of whether the genes associated with individual variation in HDID-1 mice are different from those associated with selection (risk) (Iancu et al., 2013). Thirty-three HDID-1 mice were phenotyped for their BECs at the end of a standard DID trial, were sacrificed 3 weeks later, and RNA-Seq was used to analyze the striatal transcriptome. The data obtained illustrate that there is considerable overlap of the risk and variation gene sets, both focused on the fine-tuning of synaptic plasticity.

Original languageEnglish (US)
JournalAlcohol
Volume60
DOIs
StatePublished - May 1 2017

Fingerprint

Transcriptome
Drinking
Animals
Ethanol
animal
Blood
Genes
Corpus Striatum
Neuronal Plasticity
Gene Expression Profiling
Epigenomics
Plasticity
Tuning
RNA
Phenotype

Keywords

  • Excessive ethanol consumption
  • Individual variation
  • Risk

ASJC Scopus subject areas

  • Health(social science)
  • Medicine(all)
  • Biochemistry
  • Toxicology
  • Neurology
  • Behavioral Neuroscience

Cite this

Alignment of the transcriptome with individual variation in animals selectively bred for High Drinking-In-the-Dark (HDID). / Hitzemann, Robert; Oberbeck, Denesa; Iancu, Ovidiu; Darakjian, Priscila; McWeeney, Shannon; Spence, Stephanie; Schlumbohm, Jason; Metten, Pamela; Crabbe, John Jr.

In: Alcohol, Vol. 60, 01.05.2017.

Research output: Contribution to journalArticle

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AU - Darakjian, Priscila

AU - McWeeney, Shannon

AU - Spence, Stephanie

AU - Schlumbohm, Jason

AU - Metten, Pamela

AU - Crabbe, John Jr

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