Abstract
We describe a method for producing high-resolution chemical patterns on surfaces to control the attachment and growth of cultured neurons. Microcontact printing has been extended to allow the printing of μm-scale protein lines aligned to an underlying pattern of planar microelectrodes. Poly-L-lysine (PL) lines have been printed on the electrode array for electrical studies on cultured neural networks. Rat hippocampal neurons showed a high degree of attachment selectivity to the PL and produced neurites that faithfully grew onto the electrode recording sites.
Original language | English (US) |
---|---|
Pages (from-to) | 17-21 |
Number of pages | 5 |
Journal | IEEE Transactions on Biomedical Engineering |
Volume | 47 |
Issue number | 1 |
DOIs | |
State | Published - 2000 |
Keywords
- Hippocampal neuron culture
- Microcontact printing (μCP)
- Patterned protein
- Planar microelectrode
ASJC Scopus subject areas
- Biomedical Engineering