Aligned microcontact printing of micrometer-scale poly-L-lysine structures for controlled growth of cultured neurons on planar microelectrode arrays

C. D. James, R. Davis, M. Meyer, A. Turner, S. Turner, G. Withers, L. Kam, G. Banker, H. Craighead, M. Isaacson, J. Turner, W. Shain

Research output: Contribution to journalArticle

168 Scopus citations

Abstract

We describe a method for producing high-resolution chemical patterns on surfaces to control the attachment and growth of cultured neurons. Microcontact printing has been extended to allow the printing of μm-scale protein lines aligned to an underlying pattern of planar microelectrodes. Poly-L-lysine (PL) lines have been printed on the electrode array for electrical studies on cultured neural networks. Rat hippocampal neurons showed a high degree of attachment selectivity to the PL and produced neurites that faithfully grew onto the electrode recording sites.

Original languageEnglish (US)
Pages (from-to)17-21
Number of pages5
JournalIEEE Transactions on Biomedical Engineering
Volume47
Issue number1
DOIs
StatePublished - Jan 24 2000

Keywords

  • Hippocampal neuron culture
  • Microcontact printing (μCP)
  • Patterned protein
  • Planar microelectrode

ASJC Scopus subject areas

  • Biomedical Engineering

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    James, C. D., Davis, R., Meyer, M., Turner, A., Turner, S., Withers, G., Kam, L., Banker, G., Craighead, H., Isaacson, M., Turner, J., & Shain, W. (2000). Aligned microcontact printing of micrometer-scale poly-L-lysine structures for controlled growth of cultured neurons on planar microelectrode arrays. IEEE Transactions on Biomedical Engineering, 47(1), 17-21. https://doi.org/10.1109/10.817614