Alcohol drinking produces brain region-selective changes in expression of inducible transcription factors

Ryan K. Bachtell, Yuan Mei Wang, Pierre Freeman, Fred O. Risinger, Andrey Ryabinin

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Mapping the effects of alcohol consumption on neural activity could provide valuable information on mechanisms of alcohol's effects on behavior. The present study sought to identify effects of alcohol consumption on expression of inducible transcription factors (ITFs) in mouse brain. C57BL/6J mice were trained to consume 10% ethanol/10% sucrose solution during a 30-min limited access period. Control animals were given access to 10% sucrose solution or water. Following the final day of the procedure, animals were sacrificed and immunohistochemical analyses were performed for three ITFs (c- Fos, FosB, and Zif268). Alcohol-consuming animals had increased ITF expression in several brain areas. Specifically, c-Fos was significantly induced in the nucleus accumbens core (AcbC), the medial posteroventral portion of the central nucleus of the amygdala (CeMPV), and the Edinger- Westphal nucleus (EW). Expression of c-Fos was significantly lower in the dentate gyrus of alcohol-consuming animals vs. sucrose-consuming animals. However, it was not significantly different from the water controls. Induction of c-Fos in AcbC, CeMPV and EW was significantly related to blood alcohol concentrations (BAC). Furthermore, FosB expression in the CeMPV and the EW was also significantly higher in the alcohol-consuming animals vs. water controls. FosB expression in the EW was significantly related to BAC. The significance of these results is two-fold. First, our experiments demonstrate that ITF mapping is an effective strategy in identifying alcohol- induced changes following voluntary consumption. Second, they suggest a relationship between ITF expression in AcbC, CeMPV and EW and the level of alcohol intoxication.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalBrain Research
Volume847
Issue number2
DOIs
StatePublished - Nov 20 1999
Externally publishedYes

Fingerprint

Alcohol Drinking
Transcription Factors
Alcohols
Brain
Sucrose
Water
Alcoholic Intoxication
Dentate Gyrus
Nucleus Accumbens
Inbred C57BL Mouse
Ethanol
Edinger-Westphal Nucleus
Blood Alcohol Content

Keywords

  • c-Fos
  • Edinger-Westphal
  • Ethanol
  • FosB
  • Zif268

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Alcohol drinking produces brain region-selective changes in expression of inducible transcription factors. / Bachtell, Ryan K.; Wang, Yuan Mei; Freeman, Pierre; Risinger, Fred O.; Ryabinin, Andrey.

In: Brain Research, Vol. 847, No. 2, 20.11.1999, p. 157-165.

Research output: Contribution to journalArticle

Bachtell, Ryan K. ; Wang, Yuan Mei ; Freeman, Pierre ; Risinger, Fred O. ; Ryabinin, Andrey. / Alcohol drinking produces brain region-selective changes in expression of inducible transcription factors. In: Brain Research. 1999 ; Vol. 847, No. 2. pp. 157-165.
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