Age-related decreases in SYN levels associated with increases in MAP-2, apoE, and GFAP levels in the rhesus macaque prefrontal cortex and hippocampus

Gwendolen E. Haley, Steven Kohama, Henryk Urbanski, Jacob Raber

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Loss of synaptic integrity in the hippocampus and prefrontal cortex (PFC) may play an integral role in age-related cognitive decline. Previously, we showed age-related increases in the dendritic marker microtubule associated protein 2 (MAP-2) and the synaptic marker synaptophysin (SYN) in mice. Similarly, apolipoprotein E (apoE), involved in lipid transport and metabolism, and glial fibrillary acidic protein (GFAP), a glia specific marker, increase with age in rodents. In this study, we assessed whether these four proteins show similar age-related changes in a nonhuman primate, the rhesus macaque. Free-floating sections from the PFC and hippocampus from adult, middle-aged, and aged rhesus macaques were immunohistochemically labeled for MAP-2, SYN, apoE, and GFAP. Protein levels were measured as area occupied by fluorescence using confocal microscopy as well as by Western blot. In the PFC and hippocampus of adult and middle-aged animals, the levels of SYN, apoE, and GFAP immunoreactivity were comparable but there was a trend towards higher MAP-2 levels in middle-aged than adult animals. There was significantly less SYN and more MAP-2, apoE, and GFAP immunoreactivity in the PFC and hippocampus of aged animals compared to adult or middle-aged animals. Thus, the age-related changes in MAP-2, apoE, and GFAP levels were similar to those previously observed in rodents. On the other hand, the age-related changes in SYN levels were not, but were similar to those previously observed in the aging human brain. Taken together, these data emphasize the value of the rhesus macaque as a pragmatic translational model for human brain aging.

Original languageEnglish (US)
Pages (from-to)283-296
Number of pages14
JournalAge
Volume32
Issue number3
DOIs
StatePublished - Sep 2010

Fingerprint

Synaptophysin
Microtubule-Associated Proteins
Glial Fibrillary Acidic Protein
Apolipoproteins E
Prefrontal Cortex
Macaca mulatta
Hippocampus
Rodentia
Brain
Lipid Metabolism
Confocal Microscopy
Neuroglia
Primates
Proteins
Fluorescence
Western Blotting

Keywords

  • Aging
  • ApoE
  • MAP-2
  • Nonhuman primate
  • Synaptophysin

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Age-related decreases in SYN levels associated with increases in MAP-2, apoE, and GFAP levels in the rhesus macaque prefrontal cortex and hippocampus. / Haley, Gwendolen E.; Kohama, Steven; Urbanski, Henryk; Raber, Jacob.

In: Age, Vol. 32, No. 3, 09.2010, p. 283-296.

Research output: Contribution to journalArticle

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