Age-related cochlear cytokine gene expression in the BALB/cJ mouse with systemic versus intratympanic dosing of steroid drugs

Sara A. Tokarz, Jiaqing Pang, Anna Grosz, J. Beth Kempton, Dennis R. Trune, De Ann M. Pillers

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Conclusion: Age-related differences in the expression of inflammatory cytokines in the inner ear may contribute to the development of age-related hearing loss (ARHL). Objectives: ARHL is characterized by tissue remodeling, ischemia, ion homeostasis, and inflammation. Steroid therapy is an otoprotective strategy that likely acts by reducing inflammation. We examined age-related changes in cytokine gene expression in the cochlea of the BALB/cJ mouse model of premature ARHL after systemic or intratympanic steroid delivery. Methods: 'Young' (2.5-3 months) and 'Old' (5-9 months) mice were treated with dexamethasone or fludrocortisone administered either orally or intratympanically. Cytokine gene expression in cochlear RNA was analyzed using prefabricated cDNA arrays. Old groups were compared to Young groups to identify age-related changes. Results: Down-regulation of a cytokine associated with bone remodeling (SPP1) was observed in the untreated Old group. Numerous genes were up-or down-regulated by more than twofold by steroid treatment, including proinflammatory interleukins (IL-16) and anti-inflammatory cytokines.

Original languageEnglish (US)
Pages (from-to)685-691
Number of pages7
JournalActa Oto-Laryngologica
Volume133
Issue number7
DOIs
StatePublished - Jul 2013

Keywords

  • Aging
  • Animal model
  • Glucocorticoid
  • Inflammation
  • Inner ear
  • Mineralocorticoid
  • SPP1
  • Sensorineural hearing loss

ASJC Scopus subject areas

  • Otorhinolaryngology

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