Age-related changes of alpha-crystallin aggregate in human lens

N. Fujii, Y. Shimmyo, M. Sakai, Y. Sadakane, T. Nakamura, Y. Morimoto, T. Kinouchi, Y. Goto, Kirsten Lampi

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Lens alpha-crystallin, composed of two subunits alpha A- and alpha B-crystallin, forms large aggregates in the lens of the eye. The present study investigated the aggregate of human lens alpha-crystallin from elderly and young donors. Recombinant alpha A- and alpha B-crystallins in molar ratios of alpha A to alpha B at 1:1, corresponding to the aged sample, were also studied in detail. We found by ultra-centrifugation analysis that the alpha-crystallin aggregate from elderly donors was large and heterogeneous with an average sedimentation coefficient of 30 S and a range of 20-60 S at 37 °C. This was higher compared to the young samples that had an average sedimentation coefficient of 17 S. The sedimentation coefficients of recombinant alpha A- and alpha B-crystallins were approximately 12 S and 15 S, respectively. Even when recombinant alpha-crystallins were mixed in molar ratios equivalent to those found in vivo, similar S values as the native aged alpha-crystallin aggregates were not obtained. Changes in the self-association of alpha-crystallin aggregate were correlated to changes in chaperone activity. Alpha-crystallin from young donors, and recombinant alpha A- and alpha B-crystallin and their mixtures showed chaperone activity, which was markedly lost in samples from the aged alpha-crystallin aggregates.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalAmino Acids
Volume32
Issue number1
DOIs
StatePublished - Jan 2007

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alpha-Crystallins
Lenses
alpha-Crystallin B Chain
Sedimentation
Tissue Donors
Crystalline Lens
Centrifugation

Keywords

  • Aggregation
  • Aging
  • Analytical ultracentrifugation
  • Lens alpha-crystallin

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

Cite this

Fujii, N., Shimmyo, Y., Sakai, M., Sadakane, Y., Nakamura, T., Morimoto, Y., ... Lampi, K. (2007). Age-related changes of alpha-crystallin aggregate in human lens. Amino Acids, 32(1), 87-94. https://doi.org/10.1007/s00726-006-0303-4

Age-related changes of alpha-crystallin aggregate in human lens. / Fujii, N.; Shimmyo, Y.; Sakai, M.; Sadakane, Y.; Nakamura, T.; Morimoto, Y.; Kinouchi, T.; Goto, Y.; Lampi, Kirsten.

In: Amino Acids, Vol. 32, No. 1, 01.2007, p. 87-94.

Research output: Contribution to journalArticle

Fujii, N, Shimmyo, Y, Sakai, M, Sadakane, Y, Nakamura, T, Morimoto, Y, Kinouchi, T, Goto, Y & Lampi, K 2007, 'Age-related changes of alpha-crystallin aggregate in human lens', Amino Acids, vol. 32, no. 1, pp. 87-94. https://doi.org/10.1007/s00726-006-0303-4
Fujii N, Shimmyo Y, Sakai M, Sadakane Y, Nakamura T, Morimoto Y et al. Age-related changes of alpha-crystallin aggregate in human lens. Amino Acids. 2007 Jan;32(1):87-94. https://doi.org/10.1007/s00726-006-0303-4
Fujii, N. ; Shimmyo, Y. ; Sakai, M. ; Sadakane, Y. ; Nakamura, T. ; Morimoto, Y. ; Kinouchi, T. ; Goto, Y. ; Lampi, Kirsten. / Age-related changes of alpha-crystallin aggregate in human lens. In: Amino Acids. 2007 ; Vol. 32, No. 1. pp. 87-94.
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