Age-associated gene expression changes in the arcuate nucleus of male rhesus macaques

Dominique H. Eghlidi, Vasilios T. Garyfallou, Steven Kohama, Henryk Urbanski

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    The hypothalamic arcuate nucleus (ARC) represents a major component of the neuroendocrine reproductive axis and plays an important role in controlling the onset of puberty as well as age-associated reproductive senescence. Although significant gene expression changes have been observed in the ARC during sexual maturation, it is unclear what changes occur during aging, especially in males. Therefore, in the present study, we profiled the expression of reproduction-related genes in the ARC of young and old male rhesus macaques, as well as old males that had received 6 months of hormone supplementation (HS) in the form of daily testosterone and dehydroepiandrosterone; we also compared morning vs night ARC gene expression in the old males. Using Affymetrix gene microarrays, we found little evidence for age-associated expression changes for genes associated with the neuroendocrine reproductive axis, whereas using qRT-PCR, we detected a similar age-associated decrease in PGR (progesterone receptor) that we previously observed in postmenopausal females. We also detected a sex-steroid-dependent and age-associated decrease in androgen receptor (AR) expression, with highest AR levels being expressed at night (i.e., coinciding with the natural peak in daily testosterone secretion). Finally, unlike previous observations made in females, we did not find a significant age-associated increase in KISS1 (Kisspeptin) or TAC3 (Neurokinin B) expression in the ARC of males, most likely because the attenuation of circulating sex-steroid levels in the males was much less than that in postmenopausal females. Taken together, the data highlight some similarities and differences in ARC gene expression between aged male and female nonhuman primates.

    Original languageEnglish (US)
    Pages (from-to)141-149
    Number of pages9
    JournalJournal of Molecular Endocrinology
    Volume59
    Issue number2
    DOIs
    StatePublished - Aug 1 2017

    Fingerprint

    Arcuate Nucleus of Hypothalamus
    Macaca mulatta
    Gene Expression
    Androgen Receptors
    Testosterone
    Neurokinin B
    Steroids
    Kisspeptins
    Sexual Maturation
    Dehydroepiandrosterone
    Progesterone Receptors
    Puberty
    Primates
    Genes
    Reproduction
    Hormones
    Polymerase Chain Reaction

    Keywords

    • aging
    • androgen receptor
    • circadian rhythms
    • reproduction
    • testosterone

    ASJC Scopus subject areas

    • Molecular Biology
    • Endocrinology

    Cite this

    Age-associated gene expression changes in the arcuate nucleus of male rhesus macaques. / Eghlidi, Dominique H.; Garyfallou, Vasilios T.; Kohama, Steven; Urbanski, Henryk.

    In: Journal of Molecular Endocrinology, Vol. 59, No. 2, 01.08.2017, p. 141-149.

    Research output: Contribution to journalArticle

    Eghlidi, Dominique H. ; Garyfallou, Vasilios T. ; Kohama, Steven ; Urbanski, Henryk. / Age-associated gene expression changes in the arcuate nucleus of male rhesus macaques. In: Journal of Molecular Endocrinology. 2017 ; Vol. 59, No. 2. pp. 141-149.
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