Affinity labeling of rabbit antisaccharide antibodies

Leon Wofsy, John Kimura, David H. Bing, David Parker

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Abstract

Rabbit antihapten antibodies against p-azophenyl β-lactoside and p-azophenyl β-galactoside determinants have been studied with diazoniumphenyl glycosides as affinity labeling reagents. Specific labeling of the combining regions of both antisaccharide antibodies has been demonstrated by the fact that a reversibly binding hapten affords clear-cut protection against the particular labeling reaction. As in earlier studies with several antibenzenoid hapten antibodies, both H and L chains are labeled. Azo spectra of H chains from both labeled antisaccharide antibodies show selective labeling at tyrosine residues; the L chains are characterized by a discrete visible spectrum which is markedly different from that of azotyrosine, azohistidine, or azolysine, the modified residues usually identifiable in azoproteins. While the affinity labeling patterns for both antisaccharide antibodies are identical, they are clearly distinguishable from those obtained in the antibenzenoid hapten systems which reveal specifically labeled tyrosine residues on both H and L chains.

Original languageEnglish (US)
Pages (from-to)1981-1988
Number of pages8
JournalBiochemistry
Volume6
Issue number7
Publication statusPublished - 1967
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Wofsy, L., Kimura, J., Bing, D. H., & Parker, D. (1967). Affinity labeling of rabbit antisaccharide antibodies. Biochemistry, 6(7), 1981-1988.