Affinity labeling of anti-group a streptococcal polysaccharide antibodies

David C. Parker, Richard M. Krause, Leon Wofsy

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The active sites of rabbit antibodies to the Group A streptococcal polysaccharide have been affinity labeled with a reagent (p-diazoniumphenyl-β-N-acetylglucosaminide) based on the terminal immunodominant monosaccharide of the Group A polysaccharide. Isoelectric focusing of radiolabeled antibodies showed that two of six antipolysaccharide antisera contained small amounts of restricted individual antibody components which affinity labeled with very high specificity. Most of the antibodies, including major restricted components which stand out from a heterogeneous background in isoelectric focusing, were affinity labeled with low specificit. The antipolysaccharide antibodies were purified by affinity chromatography on Sepharose conjugated with p-aminophenyl-β-N-acetylglucosaminide.

Original languageEnglish (US)
Pages (from-to)727-734
Number of pages8
JournalImmunochemistry
Volume10
Issue number11
DOIs
StatePublished - Nov 1973

ASJC Scopus subject areas

  • Medicine(all)

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