Advantages of a modified toluidine blue O stain and bronchoalveolar lavage for the diagnosis of Pneumocystis carinii pneumonia

L. L. Gosey, R. M. Howard, F. G. Witebsky, F. P. Ognibene, T. C. Wu, V. J. Gill, J. D. MacLowry

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

A modified toluidine blue O staining technique for Pneumocystis carinii is described. An easily prepared sulfation reagent made with sulfuric and acetic acids was used. The stain can be employed for broncheoalveolar lavages and lung tissue touch preparations. Most background material was removed by the sulfation reagent, slides were generally easy to read, and time from receipt of a specimen to reporting of results was approximately 1 h. P. carinii cysts were more easily visualized with this stain than in slides stained with modified methylene blue, Gram, Gram-Weigert, and standard toluidine blue O procedures. The importance of certain procedural aspects of subsegmental broncheoalveolar lavage, by which most of the specimens were obtained, is also emphasized.

Original languageEnglish (US)
Pages (from-to)803-807
Number of pages5
JournalJournal of Clinical Microbiology
Volume22
Issue number5
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Microbiology (medical)

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