Advances in visualization of copper in mammalian systems using X-ray fluorescence microscopy

Scot C. Leary; Martina Ralle

doi:10.1016/j.cbpa.2019.12.002

Advances in visualization of copper in mammalian systems using X-ray fluorescence microscopy

Scot C. Leary, Martina Ralle

Molecular and Medical Genetics

Research output: Contribution to journal › Review article › peer-review

17 Scopus citations

Abstract

Synchrotron-based X-ray fluorescence microscopy (XFM) has become an important imaging technique to investigate elemental concentrations and distributions in biological specimens. Advances in technology now permit imaging at resolutions rivaling that of electron microscopy, and researchers can now visualize elemental concentrations in subcellular organelles when using appropriate correlative methods. XFM is an especially valuable tool to determine the distribution of endogenous trace metals that are involved in neurodegenerative diseases. Here, we discuss the latest research on the unusual copper (Cu) storage vesicles that were originally identified in mouse brains and the involvement of Cu in Alzheimer's disease. Finally, we provide an outlook of how future improvements to XFM will drive current trace element research forward.

Original language	English (US)
Pages (from-to)	19-25
Number of pages	7
Journal	Current Opinion in Chemical Biology
Volume	55
DOIs	https://doi.org/10.1016/j.cbpa.2019.12.002
State	Published - Apr 2020

Keywords

Copper dyshomeostasis in Alzheimer's disease
Copper storage vesicles
X-ray fluorescence microscopy

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1016/j.cbpa.2019.12.002

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abstract = "Synchrotron-based X-ray fluorescence microscopy (XFM) has become an important imaging technique to investigate elemental concentrations and distributions in biological specimens. Advances in technology now permit imaging at resolutions rivaling that of electron microscopy, and researchers can now visualize elemental concentrations in subcellular organelles when using appropriate correlative methods. XFM is an especially valuable tool to determine the distribution of endogenous trace metals that are involved in neurodegenerative diseases. Here, we discuss the latest research on the unusual copper (Cu) storage vesicles that were originally identified in mouse brains and the involvement of Cu in Alzheimer's disease. Finally, we provide an outlook of how future improvements to XFM will drive current trace element research forward.",

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AB - Synchrotron-based X-ray fluorescence microscopy (XFM) has become an important imaging technique to investigate elemental concentrations and distributions in biological specimens. Advances in technology now permit imaging at resolutions rivaling that of electron microscopy, and researchers can now visualize elemental concentrations in subcellular organelles when using appropriate correlative methods. XFM is an especially valuable tool to determine the distribution of endogenous trace metals that are involved in neurodegenerative diseases. Here, we discuss the latest research on the unusual copper (Cu) storage vesicles that were originally identified in mouse brains and the involvement of Cu in Alzheimer's disease. Finally, we provide an outlook of how future improvements to XFM will drive current trace element research forward.

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Advances in visualization of copper in mammalian systems using X-ray fluorescence microscopy

Abstract

Keywords

ASJC Scopus subject areas

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