Adoptive transfer of lymphocytes isolated from simian immunodeficiency virus SIVmac239δnef-vaccinated macaques does not affect acute-phase viral loads but may reduce chronic-phase viral loads in major histocompatibility complex-matched recipients

Justin M. Greene, Jennifer J. Lhost, Paul J. Hines, Matthew Scarlotta, Max Harris, Benjamin Burwitz, Melisa L. Budde, Dawn M. Dudley, Ngoc Pham, Brian Cain, Caitlin E. Mac Nair, Madelyn K. Weiker, Shelby L. O'Connor, Thomas C. Friedrich, David H. O'Connor

Research output: Contribution to journalArticle

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Abstract

The live attenuated simian immunodeficiency virus (SIV) SIVmac239Δnef is the most effective SIV/human immunodeficiency virus (HIV) vaccine in preclinical testing. An understanding of the mechanisms responsible for protection may provide important insights for the development of HIV vaccines. Leveraging the uniquely restricted genetic diversity of Mauritian cynomolgus macaques, we performed adoptive transfers between major histocompatibility complex (MHC)-matched animals to assess the role of cellular immunity in SIVmac239δnef protection. We vaccinated and mock vaccinated donor macaques and then harvested between 1.25 ×109 and 3.0×109 mononuclear cells from multiple tissues for transfer into 12 naive recipients, followed by challenge with pathogenic SIVmac239. Fluorescently labeled donor cells were detectable for at least 7 days posttransfer and trafficked to multiple tissues, including lung, lymph nodes, and other mucosal tissues. There was no difference between recipient macaques' peak or postpeak plasma viral loads. A very modest difference in viral loads during the chronic phase between vaccinated animal cell recipients and mock-vaccinated animal cell recipients did not reach significance (P=0.12). Interestingly, the SIVmac239 challenge virus accumulated escape mutations more rapidly in animals that received cells from vaccinated donors. These results may suggest that adoptive transfers influenced the course of infection despite the lack of significant differences in the viral loads among animals that received cells from vaccinated and mock-vaccinated donor animals.

Original languageEnglish (US)
Pages (from-to)7382-7392
Number of pages11
JournalJournal of Virology
Volume87
Issue number13
DOIs
StatePublished - Jul 2013

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Adoptive Transfer
Macaca
major histocompatibility complex
viral load
Major Histocompatibility Complex
Viral Load
lymphocytes
Lymphocytes
animals
Human immunodeficiency virus
cells
Vaccines
HIV
vaccines
Macaca fascicularis
Cellular Immunity
cell-mediated immunity
lymph nodes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Adoptive transfer of lymphocytes isolated from simian immunodeficiency virus SIVmac239δnef-vaccinated macaques does not affect acute-phase viral loads but may reduce chronic-phase viral loads in major histocompatibility complex-matched recipients. / Greene, Justin M.; Lhost, Jennifer J.; Hines, Paul J.; Scarlotta, Matthew; Harris, Max; Burwitz, Benjamin; Budde, Melisa L.; Dudley, Dawn M.; Pham, Ngoc; Cain, Brian; Mac Nair, Caitlin E.; Weiker, Madelyn K.; O'Connor, Shelby L.; Friedrich, Thomas C.; O'Connor, David H.

In: Journal of Virology, Vol. 87, No. 13, 07.2013, p. 7382-7392.

Research output: Contribution to journalArticle

Greene, JM, Lhost, JJ, Hines, PJ, Scarlotta, M, Harris, M, Burwitz, B, Budde, ML, Dudley, DM, Pham, N, Cain, B, Mac Nair, CE, Weiker, MK, O'Connor, SL, Friedrich, TC & O'Connor, DH 2013, 'Adoptive transfer of lymphocytes isolated from simian immunodeficiency virus SIVmac239δnef-vaccinated macaques does not affect acute-phase viral loads but may reduce chronic-phase viral loads in major histocompatibility complex-matched recipients', Journal of Virology, vol. 87, no. 13, pp. 7382-7392. https://doi.org/10.1128/JVI.00348-13
Greene, Justin M. ; Lhost, Jennifer J. ; Hines, Paul J. ; Scarlotta, Matthew ; Harris, Max ; Burwitz, Benjamin ; Budde, Melisa L. ; Dudley, Dawn M. ; Pham, Ngoc ; Cain, Brian ; Mac Nair, Caitlin E. ; Weiker, Madelyn K. ; O'Connor, Shelby L. ; Friedrich, Thomas C. ; O'Connor, David H. / Adoptive transfer of lymphocytes isolated from simian immunodeficiency virus SIVmac239δnef-vaccinated macaques does not affect acute-phase viral loads but may reduce chronic-phase viral loads in major histocompatibility complex-matched recipients. In: Journal of Virology. 2013 ; Vol. 87, No. 13. pp. 7382-7392.
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AU - Harris, Max

AU - Burwitz, Benjamin

AU - Budde, Melisa L.

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AU - Cain, Brian

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