Administration of daniplestim and granulocyte colony-stimulating factor for the mobilization of hematopoietic progenitor cells in nonhuman primates

William H. Fleming, Pamela Lankford-Turner, Curtis W. Turner, John Wong, Elizabeth Strobert, John P. McKearn

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We investigated the effects of administering a high-affinity interleukin-3 receptor agonist (daniplestim) and granulocyte colony-stimulating factor (G-CSF) on the mobilization of primitive hematopoietic progenitor cells into peripheral blood (PB). Groups of five rhesus monkeys were treated with 100 mg/kg of daniplestim for 5 days followed by 10 μg/kg of G-CSF for 5 days (D/G), daniplestim and G-CSF administered concurrently for 10 days (D+G), or G-CSF alone for 10 days. Phenotypic PB analysis indicated that the number of CD34+ cells in the G-CSF group had increased to 28x106/L by Day 3 and then declined. In contrast, CD34+ cell counts of up to 68x106/L were maintained until Day 10 in both the D/G and D+G groups. On Day 5, the total number of colony-forming cells in the PB had increased 15-fold in the D+G group and eightfold in both the D/G group and the G-CSF groups. By Day 7, the numbers of colony-forming units granulocyte/macrophage were comparable in all three groups, and 45-fold increases in the numbers of burst-forming units-erythroid and 12-fold increases in the numbers of multipotent colony-forming units were seen in both the D+G and the D/G groups. The frequency of circulating primitive progenitor cells in long-term stromal cultures was highest with D+G and lowest with G-CSF alone. These results indicate that the combination of daniplestim and G-CSF produces higher and more sustained levels of circulating stem cells than does G-CSF alone. D+G may offer advantages over D/G because it generates more long- and short-term clonogenic cells.

Original languageEnglish (US)
Pages (from-to)8-14
Number of pages7
JournalBiology of Blood and Marrow Transplantation
Volume5
Issue number1
DOIs
StatePublished - Jan 1 1999

Keywords

  • G-CSF
  • IL-3
  • Peripheral blood stem cells
  • Rhesus
  • Transplantation

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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