Adipose tissue NK cells manifest an activated phenotype in human obesity

Robert W. O'Rourke; Garen D. Gaston; Kevin A. Meyer; Ashley E. White; Daniel L. Marks

doi:10.1016/j.metabol.2013.07.011

Adipose tissue NK cells manifest an activated phenotype in human obesity

Robert W. O'Rourke, Garen D. Gaston, Kevin A. Meyer, Ashley E. White, Daniel L. Marks

Pediatrics

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49 Scopus citations

Abstract

Objective Adipose tissue inflammation is a cause of obesity-related metabolic disease. Natural killer (NK) cells are an understudied cell type in the context of obesity. The goal of this study was to determine the phenotype of human adipose tissue NK cells. Methods We used flow cytometry phenotyping to study adipose tissue and peripheral blood NK cells from obese and lean humans. Results Human adipose tissue NK cells, relative to peripheral blood NK cells, express increased levels of activation markers. Adipose tissue NK cells also demonstrate an activated phenotype in obese relative to lean subjects, with increased expression of the activating receptor NKG2D. Conclusions These data are the first detailed phenotypic characterization of human adipose tissue NK cells, and suggest a role for NK cells in adipose tissue inflammation in obesity.

Original language	English (US)
Pages (from-to)	1557-1561
Number of pages	5
Journal	Metabolism: Clinical and Experimental
Volume	62
Issue number	11
DOIs	https://doi.org/10.1016/j.metabol.2013.07.011
State	Published - Nov 2013

Keywords

CD56
Inflammation
NKG2D

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1016/j.metabol.2013.07.011

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title = "Adipose tissue NK cells manifest an activated phenotype in human obesity",

abstract = "Objective Adipose tissue inflammation is a cause of obesity-related metabolic disease. Natural killer (NK) cells are an understudied cell type in the context of obesity. The goal of this study was to determine the phenotype of human adipose tissue NK cells. Methods We used flow cytometry phenotyping to study adipose tissue and peripheral blood NK cells from obese and lean humans. Results Human adipose tissue NK cells, relative to peripheral blood NK cells, express increased levels of activation markers. Adipose tissue NK cells also demonstrate an activated phenotype in obese relative to lean subjects, with increased expression of the activating receptor NKG2D. Conclusions These data are the first detailed phenotypic characterization of human adipose tissue NK cells, and suggest a role for NK cells in adipose tissue inflammation in obesity.",

keywords = "CD56, Inflammation, NKG2D",

author = "O'Rourke, {Robert W.} and Gaston, {Garen D.} and Meyer, {Kevin A.} and White, {Ashley E.} and Marks, {Daniel L.}",

note = "Funding Information: This work is supported by National Institutes of Health Grants K08DK074397 (RWO), R03DK095050 (RWO), R01DK097449 (RWO), and R01DK070333 (DLM) . ",

year = "2013",

month = nov,

doi = "10.1016/j.metabol.2013.07.011",

language = "English (US)",

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pages = "1557--1561",

journal = "Metabolism: Clinical and Experimental",

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T1 - Adipose tissue NK cells manifest an activated phenotype in human obesity

AU - O'Rourke, Robert W.

AU - Gaston, Garen D.

AU - Meyer, Kevin A.

AU - White, Ashley E.

AU - Marks, Daniel L.

N1 - Funding Information: This work is supported by National Institutes of Health Grants K08DK074397 (RWO), R03DK095050 (RWO), R01DK097449 (RWO), and R01DK070333 (DLM) .

PY - 2013/11

Y1 - 2013/11

N2 - Objective Adipose tissue inflammation is a cause of obesity-related metabolic disease. Natural killer (NK) cells are an understudied cell type in the context of obesity. The goal of this study was to determine the phenotype of human adipose tissue NK cells. Methods We used flow cytometry phenotyping to study adipose tissue and peripheral blood NK cells from obese and lean humans. Results Human adipose tissue NK cells, relative to peripheral blood NK cells, express increased levels of activation markers. Adipose tissue NK cells also demonstrate an activated phenotype in obese relative to lean subjects, with increased expression of the activating receptor NKG2D. Conclusions These data are the first detailed phenotypic characterization of human adipose tissue NK cells, and suggest a role for NK cells in adipose tissue inflammation in obesity.

AB - Objective Adipose tissue inflammation is a cause of obesity-related metabolic disease. Natural killer (NK) cells are an understudied cell type in the context of obesity. The goal of this study was to determine the phenotype of human adipose tissue NK cells. Methods We used flow cytometry phenotyping to study adipose tissue and peripheral blood NK cells from obese and lean humans. Results Human adipose tissue NK cells, relative to peripheral blood NK cells, express increased levels of activation markers. Adipose tissue NK cells also demonstrate an activated phenotype in obese relative to lean subjects, with increased expression of the activating receptor NKG2D. Conclusions These data are the first detailed phenotypic characterization of human adipose tissue NK cells, and suggest a role for NK cells in adipose tissue inflammation in obesity.

KW - CD56

KW - Inflammation

KW - NKG2D

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Adipose tissue NK cells manifest an activated phenotype in human obesity

Abstract

Keywords

ASJC Scopus subject areas

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