This study examined the effect of different enamel and dentin conditioning times on the shear bond strength of a resin composite using etchand-rinse and self-etch adhesive systems. Shear bond strengths were determined following treatment of flat ground human enamel and dentin surfaces (4000 grit) with 11 adhesive systems: 1) AdheSE One Viva Pen-(ASE), 2) Adper Prompt LPop-(PLP), 3) Adper Single Bond Plus-(SBP), 4) Clearfil SE Bond-(CSE), 5) Clearfil S 3 Bond-(CS3), 6) OptiBond All-In-One-(OBA), 7) OptiBond Solo Plus-(OBS), 8) Peak SE-(PSE), 9) Xeno IV-X4), 10) Xeno V-(X5) and 11) XP Bond-(XPB) using recommended treatment times and an extended treatment time of 60 seconds (n=10/group). Composite (Z100) to enamel and dentin bond strengths (24 hours) were determined using Ultradent fixtures and debonded with a crosshead speed of 1 mm/minute. The data were analyzed with a three-way Analysis of Variance (ANOVA) and Fisher's LSD post hoc test. The highest shear bond strengths (MPa) to enamel were achieved by the three etch-and-rinse systems at both the recommended treatment time (SBP-40.5 ± 6.1; XPB-38.7 ± 3.7; OBS- 35.2 ± 6.2) and the extended treatment time (SBP-44.5 ± 8.1; XPB-40.9 ± 5.7; OBS-35.0 ± 4.5). Extending the enamel treatment time did not produce a significant change (p>0.05) in bond strength for the 11 adhesive systems tested. OBS generated the highest (46.2 ± 7.9) bond strengths to dentin at the recommended treatment time. At the extended treatment time X4 (42.2 ± 11.7), PSE (42.1 ± 9.7) and OBS (41.4 ± 8.0) produced the highest bond strengths to dentin. The bond strength change between recommend and extended treatment times was significant (p<0.05) for PSE, but the other 10 systems did not exhibit any significant change.
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