Adenoviral ElA-associated protein p300 as a functional homologue of the transcriptional co-activator CBP

James R. Lundblad, Roland P.S. Kwok, Megan E. Laurance, Marian L. Harter, Richard H. Goodman

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Abstract

THE 265K nuclear protein CBP was initially identified as a co-activator for the protein kinase A (PKA)-phosphorylated form of the transcription factor CREB1. The domains in CBP that are involved in CREB binding and transcriptional activation are highly related to the adenoviral ElA-associated cellular protein p300 (refs 2, 3), and to two hypothetical proteins from Caenorhabditis elegans, R10E11.1 and K03H1.10 (refs 4 and 5, respectively), whose functions are unknown. Here, we show that CBP and p300 have similar binding affinity for the PKA-phosphorylated form of CREB, and that p300 can substitute for CBP in potentiating CREB-activated gene expression. We find that E1A binds to CBP through a domain conserved with p300 and represses the CREB-dependent co-activator functions of both CBP and p300. Our results indicate that the gene repression and cell immortalization functions associated with El A involve the inactivation of a family of related proteins that normally participate in second-messenger-regulated gene expression.

Original languageEnglish (US)
Pages (from-to)85-88
Number of pages4
JournalNature
Volume374
Issue number6517
DOIs
StatePublished - Jan 1 1995

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