Adenosine receptors mediate glutamate-evoked arteriolar dilation in the rat cerebral cortex

Jeffrey Iliff, Raimondo D'Ambrosio, Al C. Ngai, H. Richard Winn

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

We tested the hypothesis that adenosine (Ado) mediates glutamate-induced vasodilation in the cerebral cortex by monitoring pial arteriole diameter in chloralose-anesthetized rats equipped with closed cranial windows. Topical application of 100 μM glutamate and 100 μM N-methyl-D-aspartate (NMDA) dilated pial arterioles (baseline diameter 25 ± 2 μm) by 17 ± 1% and 18 ± 4%, respectively. Coapplication of the nonselective Ado receptor antagonist theophylline (Theo; 10 μM) significantly reduced glutamate- and NMDA-induced vasodilation to 4 ± 2% (P <0.01) and 6 ± 2% (P <0.05), whereas the Ado A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (0.1 μM) had no effect. Moreover, application of the Ado A2A receptor-selective antagonist 4-{2-[7-amino-2-(2-furyl)(1,2,4)triazolo(2,3-a)(1,3,5)-triazin-5- ylamino]ethyl}phenol (ZM-241385), either by superfusion (0.1 μM, 1 μM) or intravenously (1 mg/kg), significantly inhibited the pial arteriole dilation response to glutamate. Neither Theo nor ZM-241385 affected vascular reactivity to mild hypercapnia induced by 5% CO2 inhalation. These results suggest that Ado contributes to the dilation of rat cerebral arterioles induced by exogenous glutamate, and that the Ado A2A receptor subtype may be involved in this dilation response.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume284
Issue number5 53-5
StatePublished - May 1 2003
Externally publishedYes

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Purinergic P1 Receptors
Cerebral Cortex
Arterioles
Dilatation
Glutamic Acid
N-Methylaspartate
Vasodilation
Adenosine
Adenosine A2 Receptor Antagonists
Adenosine A1 Receptor Antagonists
Adenosine A2A Receptors
Purinergic P1 Receptor Antagonists
Chloralose
Hypercapnia
Theophylline
Phenol
Inhalation
Blood Vessels

Keywords

  • 4-{2-[7-amino-2-(2-furyl)(1,2,4)triazolo(2,3-a)-(1,3,5) triazin-5-ylamino]ethyl}phenol
  • Cerebral blood flow
  • Theophylline

ASJC Scopus subject areas

  • Physiology

Cite this

Adenosine receptors mediate glutamate-evoked arteriolar dilation in the rat cerebral cortex. / Iliff, Jeffrey; D'Ambrosio, Raimondo; Ngai, Al C.; Winn, H. Richard.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 284, No. 5 53-5, 01.05.2003.

Research output: Contribution to journalArticle

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