Adenine phosphoribosyltransferase-deficient Leishmania donovani.

K. Kaur, D. M. Iovannisci, Buddy Ullman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mutant promastigotes of Leishmania donovani deficient in adenine phosphoribosyltransferase (APRTase) have been isolated in medium containing 4-aminopyrazolopyrimidine. The generation of APRTase-deficient mutants occurred in two discrete steps. In the first step, clones were isolated with 50% of wildtype levels of APRTase activity. These cells were reselected and colonies totally deficient in APRTase were isolated. Partially and totally APRTase-deficient cells exhibited intermediate and complete resistance to cytotoxic adenine analogs, respectively. Nevertheless, wildtype and mutant cells could salvage adenine and utilize adenine as a purine source equally efficiently, suggesting that the adenine deaminase-HGPRTase pathway plays an important role in promastigote adenine metabolism. Kinetic and thermal inactivation studies of purified APRTase and isoelectric focusing of crude extracts from wildtype and partially APRTase-deficient cells suggested that the latter cells possessed wildtype APRTase activity at half the amount found in wildtype parental cells. These data suggest that Leishmania donovani possess two copies of the APRTase structural gene and that these organisms might be diploid for the APRTase locus.

Original languageEnglish (US)
Pages (from-to)553-557
Number of pages5
JournalAdvances in Experimental Medicine and Biology
Volume195 Pt B
StatePublished - 1986
Externally publishedYes

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Adenine Phosphoribosyltransferase
Leishmania donovani
Adenine
adenine deaminase
Salvaging
Enzyme kinetics
Isoelectric Focusing
Complex Mixtures
Diploidy
Metabolism
Clone Cells
Hot Temperature
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Adenine phosphoribosyltransferase-deficient Leishmania donovani. / Kaur, K.; Iovannisci, D. M.; Ullman, Buddy.

In: Advances in Experimental Medicine and Biology, Vol. 195 Pt B, 1986, p. 553-557.

Research output: Contribution to journalArticle

Kaur, K, Iovannisci, DM & Ullman, B 1986, 'Adenine phosphoribosyltransferase-deficient Leishmania donovani.', Advances in Experimental Medicine and Biology, vol. 195 Pt B, pp. 553-557.
Kaur, K. ; Iovannisci, D. M. ; Ullman, Buddy. / Adenine phosphoribosyltransferase-deficient Leishmania donovani. In: Advances in Experimental Medicine and Biology. 1986 ; Vol. 195 Pt B. pp. 553-557.
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