ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum: Divergent patterns and stage-dependent regulation during the natural menstrual cycle

Kelly A. Young, Barton Tumlinson, Richard Stouffer

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalioproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P <0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P <0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P <0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.

    Original languageEnglish (US)
    Pages (from-to)559-565
    Number of pages7
    JournalMolecular Human Reproduction
    Volume10
    Issue number8
    DOIs
    StatePublished - Aug 2004

    Fingerprint

    Thrombospondin 1
    Disintegrins
    Corpus Luteum
    Metalloproteases
    Menstrual Cycle
    Primates
    Steroids
    Messenger RNA
    Progesterone
    Promegestone
    Luteal Cells
    Ovulation Induction
    Granulosa Cells
    Luteal Phase
    Progestins
    Macaca mulatta

    Keywords

    • ADAMTS-1
    • Corpus luteum
    • Ovary
    • Primate
    • TIMP-3

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Genetics
    • Developmental Biology
    • Embryology
    • Cell Biology

    Cite this

    @article{9bcfab52bc864757a470d88325341d89,
    title = "ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum: Divergent patterns and stage-dependent regulation during the natural menstrual cycle",
    abstract = "Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalioproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P <0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P <0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P <0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.",
    keywords = "ADAMTS-1, Corpus luteum, Ovary, Primate, TIMP-3",
    author = "Young, {Kelly A.} and Barton Tumlinson and Richard Stouffer",
    year = "2004",
    month = "8",
    doi = "10.1093/molehr/gah079",
    language = "English (US)",
    volume = "10",
    pages = "559--565",
    journal = "Molecular Human Reproduction",
    issn = "1360-9947",
    publisher = "Oxford University Press",
    number = "8",

    }

    TY - JOUR

    T1 - ADAMTS-1/METH-1 and TIMP-3 expression in the primate corpus luteum

    T2 - Divergent patterns and stage-dependent regulation during the natural menstrual cycle

    AU - Young, Kelly A.

    AU - Tumlinson, Barton

    AU - Stouffer, Richard

    PY - 2004/8

    Y1 - 2004/8

    N2 - Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalioproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P <0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P <0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P <0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.

    AB - Studies were designed to determine if ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin repeats-1) is expressed in the rhesus monkey corpus luteum (CL), is regulated by endocrine (LH) or local (progesterone) factors, and is correlated with tissue inhibitor of matrix metalioproteinase-3 (TIMP-3), an inhibitor of ADAMTS-1. PCR analyses indicated that ADAMTS-1 mRNA is expressed in luteinized granulosa cells during controlled ovarian stimulation cycles, and peaks in CL during the early luteal phase of the menstrual cycle, before decreasing (P <0.05) by the mid-late stage. Immunostaining for ADAMTS-1 was detected in luteal cells, peaking in early CL. LH and/or steroid depletion at mid-late luteal stage decreased (P <0.05) ADAMTS-1 mRNA levels compared to controls; LH but not progestin (R5020) replacement prevented this decrease. In contrast, LH and/or steroid ablation-replacement in the early CL did not affect ADAMTS-1 levels. TIMP-3 mRNA levels were lowest during the early CL and rose progressively (P <0.05), peaking in late CL. The divergent expression patterns during the CL lifespan suggest that an imbalance between ADAMTS-1 and TIMP-3 is important during luteal formation (ADAMTS-1 predominates) and regression (TIMP-3 predominates). Also, LH, perhaps via steroids other than progesterone, promotes ADAMTS-1 expression as a function of the stage of the CL.

    KW - ADAMTS-1

    KW - Corpus luteum

    KW - Ovary

    KW - Primate

    KW - TIMP-3

    UR - http://www.scopus.com/inward/record.url?scp=4344639838&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=4344639838&partnerID=8YFLogxK

    U2 - 10.1093/molehr/gah079

    DO - 10.1093/molehr/gah079

    M3 - Article

    C2 - 15208368

    AN - SCOPUS:4344639838

    VL - 10

    SP - 559

    EP - 565

    JO - Molecular Human Reproduction

    JF - Molecular Human Reproduction

    SN - 1360-9947

    IS - 8

    ER -