Acute perilymphatic perfusion of the guinea pig cochlea

Alfred L. Nuttall, Michael J. LaRouere, Merle Lawrence

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

A method for the continuous perfusion of the perilymphatic space of the inner ear in the guinea pig is described. Artificial perilymph is supplied to the cochlea and drained away through a tubing system while flow rates from 10 μl/min to 0.3 ml/min are established by gravity syphon pressure. Techniques are also presented which allow control over the temperature of the perfusate and over the level of dissolved oxygen in the perfusate. Along with these variables, the pH of the artificial perilymph can be manipulated and various drugs can be added to the perfusate to test their effect on the inner ear. The function of the inner ear is monitored by continuous recording of the sound evoked bioelectric potentials, the cochlear microphonic and the compound action potential. The cochlear perfusion technique has many applications in the study of cochlear physiology and metabolism, and in testing the sensitivity of the inner ear to ototoxic drugs.

Original languageEnglish (US)
Pages (from-to)207-221
Number of pages15
JournalHearing Research
Volume6
Issue number2
DOIs
StatePublished - Feb 1982

Keywords

  • aminoglycoside antibiotics
  • cochlea
  • cochlear temperature
  • guinea pig
  • intracochlear oxygen tension
  • perfusion
  • perilymph
  • perilymph pH

ASJC Scopus subject areas

  • Sensory Systems

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