Acute modification of biomechanical properties of the bone-ligament insertion to rat limb unweighting

Ray Vanderby, Arthur C. Vailas, Ben K. Graf, Robert J. Thielke, Mark J. Ulm, Sean Kohles, David N. Kunz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We investigated the acute adaptation of the rat femurmedial collateral ligament-tibia (FMT) complex to 7 days of limb unweighting by means of a hind-limb suspension protocol. Male, young adult, Harlan SpragueDawley rats were randomly assigned to either control or suspended groups. Rats deprived of hind limb-to-ground contact forces had a 42% decrease in soleus muscle mass compared with the control group. Medial collateral ligament (MCL) length and cross-sectional area were measured, and each FMT complex was tension tested to failure. All failed at their tibia-MCL insertion. The ultimate load in the FMT and the peak Kirchhoff stress in the MCL (occurring immediately before insertion site failure) were significantly reduced in the suspended group. The suspended MCLs were 9.7% larger in area and 5.7% shorter in length than the controls under the same preload (0.25 N). We found no significant differences between the control and suspended MCLs in Green strain, stretch, or deformation immediately before insertion site failure, nor did we find a significant difference in the MCL tangent modulus. This study indicates that even acute periods of limb unweighting can structurally compromise bone-ligament insertions. Further, this study implies that the adaptations responsible for this structural compromise must involve acute changes in the intrinsic zone (or zones) of the bone-ligament insertion.

Original languageEnglish (US)
Pages (from-to)2499-2505
Number of pages7
JournalFASEB Journal
Volume4
Issue number8
StatePublished - May 1990
Externally publishedYes

Fingerprint

Collateral Ligaments
Ligaments
ligaments
limbs (animal)
Rats
Bone
Extremities
bones
Bone and Bones
rats
Tibia
tibia
Young Adult
Suspensions
Skeletal Muscle
young adults
Muscle
Control Groups
Loads (forces)
muscles

Keywords

  • Bone
  • Bone-ligament insertion
  • Ligaments
  • MCL
  • Mechanical properties
  • Morphology
  • Rat
  • Suspension

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Vanderby, R., Vailas, A. C., Graf, B. K., Thielke, R. J., Ulm, M. J., Kohles, S., & Kunz, D. N. (1990). Acute modification of biomechanical properties of the bone-ligament insertion to rat limb unweighting. FASEB Journal, 4(8), 2499-2505.

Acute modification of biomechanical properties of the bone-ligament insertion to rat limb unweighting. / Vanderby, Ray; Vailas, Arthur C.; Graf, Ben K.; Thielke, Robert J.; Ulm, Mark J.; Kohles, Sean; Kunz, David N.

In: FASEB Journal, Vol. 4, No. 8, 05.1990, p. 2499-2505.

Research output: Contribution to journalArticle

Vanderby, R, Vailas, AC, Graf, BK, Thielke, RJ, Ulm, MJ, Kohles, S & Kunz, DN 1990, 'Acute modification of biomechanical properties of the bone-ligament insertion to rat limb unweighting', FASEB Journal, vol. 4, no. 8, pp. 2499-2505.
Vanderby R, Vailas AC, Graf BK, Thielke RJ, Ulm MJ, Kohles S et al. Acute modification of biomechanical properties of the bone-ligament insertion to rat limb unweighting. FASEB Journal. 1990 May;4(8):2499-2505.
Vanderby, Ray ; Vailas, Arthur C. ; Graf, Ben K. ; Thielke, Robert J. ; Ulm, Mark J. ; Kohles, Sean ; Kunz, David N. / Acute modification of biomechanical properties of the bone-ligament insertion to rat limb unweighting. In: FASEB Journal. 1990 ; Vol. 4, No. 8. pp. 2499-2505.
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