Acute administration of estrogen suppresses LH secretion without altering GnRH release in ovariectomized rhesus macaques

K. Y Francis Pau, Perry M. Gliessman, David Hess, Oline Ronnekleiv, Jon E. Levine, Harold G. Spies

Research output: Contribution to journalArticle

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Abstract

The pattern of hypothalamic gonadotropin-releasing hormone (GnRH) release was examined during estrogen (E)-induced suppression of plasma luteinizing hormone (LH) in ovariectomized (OVX) rhesus macaques. In Expt. 1, 4 OVX macaques were fitted with a jugular catheter and a push-pull cannula (PPC) directed into the median eminence (ME). Push-pull perfusion (PPP) was initiated 10 h before and continued for 10 h after subcutaneous estradiol benzoate (EB) injection (42 μg/kg b.wt.). In Expt. 2, 4 additional monkeys were subjected to local intrahypothalamic perfusion with estradiol-17β (E2, 3 μM) for the last 10 h of a 20-h PPP. In Expt. 3, OVX animals were challenged with 5 μg exogenous GnRH 3 h before and 8 h after EB injection to test for changes in altered LH release. Integrated 10-min ME perfusate and intermittent 10- or 60-min peripheral plasma samples were assayed for GnRH and LH by radioimmunoassay and bioassay, respectively. In addition, 2 other OVX macaques that received similar ME-PPC placement were sacrificed 2 days after the completion of a PPP for immunocytochemical labeling of GnRH neurons at the perfusion site. The results show that after EB, hypothalamic GnRH (MBH-GnRH) release remained unaltered while LH levels declined rapidly (Expt. 1). Similarly, intrahypothalamic perfusion of E2 failed to change the pattern of MBH-GnRH release in any of 4 monkeys (Expt. 2). Conversely, plasma LH release in response to exogenous GnRH was greatly reduced after EB (Expt. 3). Immunocytochemically labeled GnRH-containing nerve fibers and cell bodies were observed adjacent to the site of PPP in the 2 monkeys examined after the completion of 20 h of PPP. Collectively, these data suggest that acute administration of E exerts immediate negative feedback effects at the pituitary through a process that is unrelated to changes in patterns of mediobasal hypothalamic GnRH release.

Original languageEnglish (US)
Pages (from-to)229-235
Number of pages7
JournalBrain Research
Volume517
Issue number1-2
DOIs
StatePublished - May 28 1990

Fingerprint

Luteinizing Hormone
Macaca mulatta
Gonadotropin-Releasing Hormone
Estrogens
Perfusion
Pituitary Hormone-Releasing Hormones
Median Eminence
Haplorhini
Macaca
Neurons
Injections
Nerve Fibers
Biological Assay
Radioimmunoassay
Estradiol
Neck
Catheters
estradiol 3-benzoate

Keywords

  • Estrogen
  • Hypothalamic gonadotropin-releasing hormone
  • Luteinizing hormone
  • Negative feedback
  • Rhesus monkey

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Acute administration of estrogen suppresses LH secretion without altering GnRH release in ovariectomized rhesus macaques. / Pau, K. Y Francis; Gliessman, Perry M.; Hess, David; Ronnekleiv, Oline; Levine, Jon E.; Spies, Harold G.

In: Brain Research, Vol. 517, No. 1-2, 28.05.1990, p. 229-235.

Research output: Contribution to journalArticle

Pau, K. Y Francis ; Gliessman, Perry M. ; Hess, David ; Ronnekleiv, Oline ; Levine, Jon E. ; Spies, Harold G. / Acute administration of estrogen suppresses LH secretion without altering GnRH release in ovariectomized rhesus macaques. In: Brain Research. 1990 ; Vol. 517, No. 1-2. pp. 229-235.
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abstract = "The pattern of hypothalamic gonadotropin-releasing hormone (GnRH) release was examined during estrogen (E)-induced suppression of plasma luteinizing hormone (LH) in ovariectomized (OVX) rhesus macaques. In Expt. 1, 4 OVX macaques were fitted with a jugular catheter and a push-pull cannula (PPC) directed into the median eminence (ME). Push-pull perfusion (PPP) was initiated 10 h before and continued for 10 h after subcutaneous estradiol benzoate (EB) injection (42 μg/kg b.wt.). In Expt. 2, 4 additional monkeys were subjected to local intrahypothalamic perfusion with estradiol-17β (E2, 3 μM) for the last 10 h of a 20-h PPP. In Expt. 3, OVX animals were challenged with 5 μg exogenous GnRH 3 h before and 8 h after EB injection to test for changes in altered LH release. Integrated 10-min ME perfusate and intermittent 10- or 60-min peripheral plasma samples were assayed for GnRH and LH by radioimmunoassay and bioassay, respectively. In addition, 2 other OVX macaques that received similar ME-PPC placement were sacrificed 2 days after the completion of a PPP for immunocytochemical labeling of GnRH neurons at the perfusion site. The results show that after EB, hypothalamic GnRH (MBH-GnRH) release remained unaltered while LH levels declined rapidly (Expt. 1). Similarly, intrahypothalamic perfusion of E2 failed to change the pattern of MBH-GnRH release in any of 4 monkeys (Expt. 2). Conversely, plasma LH release in response to exogenous GnRH was greatly reduced after EB (Expt. 3). Immunocytochemically labeled GnRH-containing nerve fibers and cell bodies were observed adjacent to the site of PPP in the 2 monkeys examined after the completion of 20 h of PPP. Collectively, these data suggest that acute administration of E exerts immediate negative feedback effects at the pituitary through a process that is unrelated to changes in patterns of mediobasal hypothalamic GnRH release.",
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