Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells

Giorgio Grasselli, Qionger He, Vivian Wan, John Adelman, Gen Ohtsuki, Christian Hansel

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The plasticity of intrinsic excitability has been described in several types of neurons, but the significance of non-synaptic mechanisms in brain plasticity and learning remains elusive. Cerebellar Purkinje cells are inhibitory neurons that spontaneously fire action potentials at high frequencies and regulate activity in their target cells in the cerebellar nuclei by generating a characteristic spike burst-pause sequence upon synaptic activation. Using patch-clamp recordings from mouse Purkinje cells, we find that depolarization-triggered intrinsic plasticity enhances spike firing and shortens the duration of spike pauses. Pause plasticity is absent from mice lacking SK2-type potassium channels (SK2-/- mice) and in occlusion experiments using the SK channel blocker apamin, while apamin wash-in mimics pause reduction. Our findings demonstrate that spike pauses can be regulated through an activity-dependent, exclusively non-synaptic, SK2 channel-dependent mechanism and suggest that pause plasticity-by altering the Purkinje cell output-may be crucial to cerebellar information storage and learning.

Original languageEnglish (US)
Pages (from-to)2546-2553
Number of pages8
JournalCell Reports
Volume14
Issue number11
DOIs
StatePublished - Mar 22 2016

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Purkinje Cells
Apamin
Plasticity
Learning
Neurons
Cerebellar Nuclei
Information Storage and Retrieval
Potassium Channels
Action Potentials
Depolarization
Clamping devices
Brain
Chemical activation
Data storage equipment
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Grasselli, G., He, Q., Wan, V., Adelman, J., Ohtsuki, G., & Hansel, C. (2016). Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells. Cell Reports, 14(11), 2546-2553. https://doi.org/10.1016/j.celrep.2016.02.054

Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells. / Grasselli, Giorgio; He, Qionger; Wan, Vivian; Adelman, John; Ohtsuki, Gen; Hansel, Christian.

In: Cell Reports, Vol. 14, No. 11, 22.03.2016, p. 2546-2553.

Research output: Contribution to journalArticle

Grasselli, G, He, Q, Wan, V, Adelman, J, Ohtsuki, G & Hansel, C 2016, 'Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells', Cell Reports, vol. 14, no. 11, pp. 2546-2553. https://doi.org/10.1016/j.celrep.2016.02.054
Grasselli G, He Q, Wan V, Adelman J, Ohtsuki G, Hansel C. Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells. Cell Reports. 2016 Mar 22;14(11):2546-2553. https://doi.org/10.1016/j.celrep.2016.02.054
Grasselli, Giorgio ; He, Qionger ; Wan, Vivian ; Adelman, John ; Ohtsuki, Gen ; Hansel, Christian. / Activity-Dependent Plasticity of Spike Pauses in Cerebellar Purkinje Cells. In: Cell Reports. 2016 ; Vol. 14, No. 11. pp. 2546-2553.
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