Activity-dependent distribution of protein kinase C-δ Within rat cerebellar Purkinje cells following unilateral labyrinthectomy

N. H. Barmack, Z. Y. Qian, H. J. Kim, J. Yoshimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Protein kinase C isoforms PKC-δand PKC-γ are expressed in Purkinje cells in the uvula-nodulus of the cerebellum. We examined the effect of Purkinje cell activity on the transcription, expression and intracellular distribution of PKC-δand PKC-γ. Relative changes in activity were induced by unilateral labyrinthectomy (UL), decreasing the activity of Purkinje cells on the side of the labyrinthectomy relative to the contralateral side with intact vestibular input. After a UL, there was decreased immunolabeling of Purkinje cell axon terminals in the ipsilateral caudal vestibular complex by antisera to PKC-δ, but not PKC-γ. Western blots prepared from the uvula-nodulus and caudal vestibular complex showed an increase in the cytosolic PKC-δand a decrease in membrane-associated PKC-δin the ipsilateral uvula-nodulus 12-48 h after the UL. Hybridization histochemistry and semiquantitative reverse transcription polymerase chain reaction (RT-PCR) demonstrated no change in transcription of PKC-δ mRNA in the uvula-nodulus 1-240 h after unilateral labyrinthectomy. We conclude that both PKC-δ and PKC-γ are constitutively expressed in Purkinje cells. The targeting of PKC-δ, but not PKC-γ, to Purkinje neuron synaptic terminals is influenced by activity.

Original languageEnglish (US)
Pages (from-to)6-20
Number of pages15
JournalExperimental Brain Research
Volume141
Issue number1
DOIs
StatePublished - 2001

Fingerprint

Purkinje Cells
Protein Kinase C
Uvula
Presynaptic Terminals
Reverse Transcription
Immune Sera
Protein Isoforms
Western Blotting
Polymerase Chain Reaction
Messenger RNA
Membranes

Keywords

  • Cerebellum
  • Nodulus
  • Protein kinase C
  • Purkinje neuron
  • Vestibular compensation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activity-dependent distribution of protein kinase C-δ Within rat cerebellar Purkinje cells following unilateral labyrinthectomy. / Barmack, N. H.; Qian, Z. Y.; Kim, H. J.; Yoshimura, J.

In: Experimental Brain Research, Vol. 141, No. 1, 2001, p. 6-20.

Research output: Contribution to journalArticle

@article{211998e34cd14b4ab83f2e239ae146a0,
title = "Activity-dependent distribution of protein kinase C-δ Within rat cerebellar Purkinje cells following unilateral labyrinthectomy",
abstract = "Protein kinase C isoforms PKC-δand PKC-γ are expressed in Purkinje cells in the uvula-nodulus of the cerebellum. We examined the effect of Purkinje cell activity on the transcription, expression and intracellular distribution of PKC-δand PKC-γ. Relative changes in activity were induced by unilateral labyrinthectomy (UL), decreasing the activity of Purkinje cells on the side of the labyrinthectomy relative to the contralateral side with intact vestibular input. After a UL, there was decreased immunolabeling of Purkinje cell axon terminals in the ipsilateral caudal vestibular complex by antisera to PKC-δ, but not PKC-γ. Western blots prepared from the uvula-nodulus and caudal vestibular complex showed an increase in the cytosolic PKC-δand a decrease in membrane-associated PKC-δin the ipsilateral uvula-nodulus 12-48 h after the UL. Hybridization histochemistry and semiquantitative reverse transcription polymerase chain reaction (RT-PCR) demonstrated no change in transcription of PKC-δ mRNA in the uvula-nodulus 1-240 h after unilateral labyrinthectomy. We conclude that both PKC-δ and PKC-γ are constitutively expressed in Purkinje cells. The targeting of PKC-δ, but not PKC-γ, to Purkinje neuron synaptic terminals is influenced by activity.",
keywords = "Cerebellum, Nodulus, Protein kinase C, Purkinje neuron, Vestibular compensation",
author = "Barmack, {N. H.} and Qian, {Z. Y.} and Kim, {H. J.} and J. Yoshimura",
year = "2001",
doi = "10.1007/s002210100855",
language = "English (US)",
volume = "141",
pages = "6--20",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Activity-dependent distribution of protein kinase C-δ Within rat cerebellar Purkinje cells following unilateral labyrinthectomy

AU - Barmack, N. H.

AU - Qian, Z. Y.

AU - Kim, H. J.

AU - Yoshimura, J.

PY - 2001

Y1 - 2001

N2 - Protein kinase C isoforms PKC-δand PKC-γ are expressed in Purkinje cells in the uvula-nodulus of the cerebellum. We examined the effect of Purkinje cell activity on the transcription, expression and intracellular distribution of PKC-δand PKC-γ. Relative changes in activity were induced by unilateral labyrinthectomy (UL), decreasing the activity of Purkinje cells on the side of the labyrinthectomy relative to the contralateral side with intact vestibular input. After a UL, there was decreased immunolabeling of Purkinje cell axon terminals in the ipsilateral caudal vestibular complex by antisera to PKC-δ, but not PKC-γ. Western blots prepared from the uvula-nodulus and caudal vestibular complex showed an increase in the cytosolic PKC-δand a decrease in membrane-associated PKC-δin the ipsilateral uvula-nodulus 12-48 h after the UL. Hybridization histochemistry and semiquantitative reverse transcription polymerase chain reaction (RT-PCR) demonstrated no change in transcription of PKC-δ mRNA in the uvula-nodulus 1-240 h after unilateral labyrinthectomy. We conclude that both PKC-δ and PKC-γ are constitutively expressed in Purkinje cells. The targeting of PKC-δ, but not PKC-γ, to Purkinje neuron synaptic terminals is influenced by activity.

AB - Protein kinase C isoforms PKC-δand PKC-γ are expressed in Purkinje cells in the uvula-nodulus of the cerebellum. We examined the effect of Purkinje cell activity on the transcription, expression and intracellular distribution of PKC-δand PKC-γ. Relative changes in activity were induced by unilateral labyrinthectomy (UL), decreasing the activity of Purkinje cells on the side of the labyrinthectomy relative to the contralateral side with intact vestibular input. After a UL, there was decreased immunolabeling of Purkinje cell axon terminals in the ipsilateral caudal vestibular complex by antisera to PKC-δ, but not PKC-γ. Western blots prepared from the uvula-nodulus and caudal vestibular complex showed an increase in the cytosolic PKC-δand a decrease in membrane-associated PKC-δin the ipsilateral uvula-nodulus 12-48 h after the UL. Hybridization histochemistry and semiquantitative reverse transcription polymerase chain reaction (RT-PCR) demonstrated no change in transcription of PKC-δ mRNA in the uvula-nodulus 1-240 h after unilateral labyrinthectomy. We conclude that both PKC-δ and PKC-γ are constitutively expressed in Purkinje cells. The targeting of PKC-δ, but not PKC-γ, to Purkinje neuron synaptic terminals is influenced by activity.

KW - Cerebellum

KW - Nodulus

KW - Protein kinase C

KW - Purkinje neuron

KW - Vestibular compensation

UR - http://www.scopus.com/inward/record.url?scp=0034778288&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034778288&partnerID=8YFLogxK

U2 - 10.1007/s002210100855

DO - 10.1007/s002210100855

M3 - Article

C2 - 11685407

AN - SCOPUS:0034778288

VL - 141

SP - 6

EP - 20

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 1

ER -